, a New Heterothallic Species of Yeast Found in Decaying Cladodes of and in Necrotic Tissue of Cereoid Cacti Free

Abstract

A new heterothallic species of has been recovered 25 times from widely separated cactus substrates. The organism has been named because the sexually most compatible strains were isolated from in Australia. Two varieties are designated based on differences in physiology, habitat, and geographic distribution. var. has a maximum temperature for growth of 30 to 33°C and assimilates citric acid strongly, but assimilation of cellobiose is latent, weak, or negative. var. grows well at 37°C, but not at 39°C; it assimilates cellobiose strongly but does not assimilate citric acid. Ecologically, var. is associated with (tribe Opuntiaeae, subtribe Opuntiinae) in Australia; var. is associated with species of the cactus tribe Pachycereeae, subtribe Pachycereinae, from various locations in the North American Sonoran Desert. A discussion of the physiological and host-plant shifts for these two varieties and three similar cactophilic yeasts is presented. The base composition of the nuclear deoxyribonucleic acid of var. (average of four strains) is 33.64 ± 0.25 mol% guanine plus cytosine and that of var. (average of 3 strains) is 33.13 ±0.23 mol% guanine plus cytosine. The type strain of and of the type variety, var. , is UCD-FS&T 77-40 (= ATCC 36836 = CBS 7010). The type strain of var. is UCD-FS&T 76-211 (= ATCC 36834 = CBS 7012).

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1979-04-01
2024-03-29
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References

  1. Barker J. S. F. 1977; Cactus-breeding Drosophila—a system for the measurement of natural selection. Proceedings of the symposium, Measuring Selection in Natural Populations, Denmark, May, 1976. Lect. Notes Biomath 19:403–430
    [Google Scholar]
  2. Barker J. S. F., Mulley J.C. 1976; Isozyme variation in natural populations of Drosophila buzzatii. Evolution 30:213–233
    [Google Scholar]
  3. Bemardi G., Foures M., Pipemo G., Sionimski P.P. 1970; Mitochondrial DNAs from respiratory-sufficient and cytoplasmic respiratory-deficient mutants of yeast. J. Mol. Biol 48:23–42
    [Google Scholar]
  4. Fowell R. R. 1969; Sporulation and hybridization of yeasts. p 363 In Rose A.H., Harrison J.S. ed The yeasts vol 1: Academic Press Inc; New York:
    [Google Scholar]
  5. Gibson A. C., Horak K.E. 1978; Systematic anatomy and phylogeny of Mexican columnar cacti. Ann. Mo. Bot. Gard 65:999
    [Google Scholar]
  6. Heed W. B., Starmer W.T., Miranda M., Miller M.W., Phaff H.J. 1976; An analysis of the yeast flora associated with cactiphilic Drosophila and their host plants in the Sonoran Desert and its relation to temperate and tropical associations. Ecology 57:151–160
    [Google Scholar]
  7. Marmur J. 1961; A procedure for the isolation of DNA from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  8. Meyer S. A., Phaff H.J. 1970; Taxonomic significance of the DNA base composition in yeast. p 1–29 In Ahearn D.G. ed Recent trends in yeast research, Spectrum vol 1: Georgia State University; Atlanta:
    [Google Scholar]
  9. Mulley J. C., Barker J. S. F. 1977; The occurrence and distribution of Drosophila aldrichi in Australia. Drosophila Inf. Serv 52:151–152
    [Google Scholar]
  10. Nakase T., Komagata K. 1970; Significance of DNA base composition in the classification of the yeast genus Pichia.. J. Gen. Appl. Microbiol 16:511–521
    [Google Scholar]
  11. Phaff H. J., Starmer Mary Miranda W. T., Miller M.W. 1978; Pichia heedii, a new species of yeast indigenous to necrotic cacti in the North American Sonoran Desert. Int. J. Syst. Bacteriol 28:326–331
    [Google Scholar]
  12. Price C. W., Fuson G.B., Phaff H.J. 1978; Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccha- romyces, Debaryomyces, and Pichia.. Microbiol. Rev 42:161–193
    [Google Scholar]
  13. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribo-nucleic acid from its buoyant density in CsCl. J. Mol. Biol 4:430–433
    [Google Scholar]
  14. Starmer W. T., Heed W.B., Miranda M., Miller M.W., Phaff H.J. 1976; The ecology of yeast flora associated with cactiphilic Drosophila and their host plants in the Sonoran desert. Microb. Ecol 3:11–30
    [Google Scholar]
  15. Starmer W. T., Phaff H.J., Mary Miranda, Miller M.W. 1978; Pichia cactophila, a new species of yeast found in decaying tissue of cacti. Int. J. Syst. Bacteriol 28:318–325
    [Google Scholar]
  16. Starmer W. T., Phaff H.J., Mary Miranda, Miller M.W. 1978; Pichia amethionina, a new hetero- thallic yeast associated with the decaying stems of cereoid cacti. Int. J. Syst. Bacteriol 28:433–441
    [Google Scholar]
  17. Szybalski W. 1969; Use of cesium sulfate for equilibrium density gradient centrifugation. Methods Enzymol 12:330–360
    [Google Scholar]
  18. van der Walt J. P. 1970; Criteria and methods used in classification. p 34–113 In Lodder J. ed The yeasts— a taxonomic study North-Holland Publishing Co; Amsterdam:
    [Google Scholar]
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