1887

Abstract

A study of the cellular and colonial morphologies, the deoxyribonucleic acid (DNA) base composition, the degree of lysis by lysozyme, and some fundamental biochemical reactions was carried out on 23 strains previously identified as members of . Included among these was NCTC 10342, the neotype strain of . The results showed that, primarily on the basis of their biochemical reactions, two of the strains belong to , two belong to , and 19 belong to . The first two strains mentioned above clearly differed from strains with respect to their DNA base composition (33.2 and 34.3 mol% guanine plus cytosine [G+C]), their lysis by lysozyme (5.6 and 6.6%), and the morphology of their macro-and microcolonies. These properties were not helpful in differentiating the other two strains from the strains, for the two strains showed high G+C values (45.9 and 47.4 mol%) and high values of lysis by lysozyme, (92.9 and 96.7%). The 19 remaining strains, confirmed as members of , had G+C contents which varied from 37.3 to 43.0 mol% and lysis percentages which varied from 67.0 to 94.9. For these strains, a significant ( < 0.05) correlation was found between the DNA base composition and lysis by lysozyme. Three biogroups were established on the basis of the biochemical reactions of the 19 strains. These biogroups are similar to the “aggregate” and the “intermediate” strains of established by Gordon et al.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-29-1-25
1979-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/29/1/ijs-29-1-25.html?itemId=/content/journal/ijsem/10.1099/00207713-29-1-25&mimeType=html&fmt=ahah

References

  1. Baptist J. N., Mandel M., Ghema R. L. 1978; Comparative zone electrophoresis of enzymes in the genus Bacillus. Int. J. Syst. Bacteriol 28:229–244
    [Google Scholar]
  2. Baumann-Grace J. B., Tomcsik J. 1957; Lyso-zymempfindlichkeit der Spezies B. megaterium. Experientia 13:148–150
    [Google Scholar]
  3. Bonde G. J., Jackson D. K. 1971; DNA-base ratios of Bacillus strains related to numerical and classical taxonomy. Proc. Soc. Gen. Microbiol 69:vii–viii
    [Google Scholar]
  4. Buttiaux R., Beerens H., Tacquet A. 1974; Manuel de techniques bacteriologiques. , 4th. ed Flammarion Medicine-Sciences; Paris:
    [Google Scholar]
  5. Candeli A., Mastrandrea V. 1964; Ricerche sullefasi dissociative del B. anthracis. Boll. Ist. Sieroter. Milan 43:45–46
    [Google Scholar]
  6. Candeli A., Mastrandrea V., Cenci G., De Bartolomeo A. 1978; Sensitivity to lytic agents and DNA base composition of several aerobic spore-bearing bacilli. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2 133:250–260
    [Google Scholar]
  7. Gibson T., Gordon R. E. 1974; Genus I. Bacillus Cohn 1872; nom. gen. cons. Nomencl. Comm. Intern. SOC. Microbiol. 1937; Opin. A. Jud. Comm. 1955, p. 529–550.. In Buchanan R. E., Gibbons N. E. ed. Bergey’s manual of determinative bacteriology. , 8th ed. The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  8. Gordon R. E. 1975; Some taxonomic observations on the genus Bacillus. Biological regulation of vectors. The saprophytic and aerobic bacteria and fungi. A conference report, Tidewater Inn, Easton, Md., October 6 to 8 DHEW publication no. (NIH) 77-1180 p 67–82 Department of Health, Education and Welfare; Washington, D.C:
    [Google Scholar]
  9. Gordon R. E., Haynes W. C., Pang C. H. N. 1973; The genus Bacillus. Agriculture handbook no. 427 U.S. Department of Agriculture; Washington, D.C:
    [Google Scholar]
  10. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  11. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol 6:109–113
    [Google Scholar]
  12. Mastrandrea V., Candeli A., De Bartolomeo A., Cenci G. 1975; Lisi cellulare e fasi dissociative del genere Bacillus. Boll. Ist. Sieroter. Milan 54:367–377
    [Google Scholar]
  13. Seki T., Chung C., Mikami H., Oshima Y. 1978; Deoxyribonucleic acid homology and taxonomy of the genus Bacillus. Int. J. Syst. Bacteriol 28:182–189
    [Google Scholar]
  14. Somerville H. S., Jones M. L. 1972; DNA competition studies within the Bacillus cereus group of bacilli. J. Gen. Microbiol 73:257–265
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-29-1-25
Loading
/content/journal/ijsem/10.1099/00207713-29-1-25
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error