The deoxyribonucleic acid base sequence similarities among 14 strains of were assessed by hybridization. Some strains produce glucan. Deoxyribonucleic acid was purified by both a conventional method and by centrifugation in CsCl. Hybridizations were done in solution with S1 nuclease and on membrane filters. The results showed two groups of moderately homologous strains. The glucan-producing strains represented a group which included some glucan-negative strains. Hybridization by the S1 method was less than with the filter method, indicating some sequence divergence within the two groups. There was a slight difference in guanine-plus-cytosine contents of the two groups.


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