1887

Abstract

The deoxyribonucleic acid base sequence similarities among 14 strains of were assessed by hybridization. Some strains produce glucan. Deoxyribonucleic acid was purified by both a conventional method and by centrifugation in CsCl. Hybridizations were done in solution with S1 nuclease and on membrane filters. The results showed two groups of moderately homologous strains. The glucan-producing strains represented a group which included some glucan-negative strains. Hybridization by the S1 method was less than with the filter method, indicating some sequence divergence within the two groups. There was a slight difference in guanine-plus-cytosine contents of the two groups.

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1978-10-01
2023-02-01
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References

  1. Andrewes F. W., Horder T. J. 1906; A study of the streptococci pathogenic for man. Lancet ii:708–713
    [Google Scholar]
  2. Barth P. T., Grinter N. J. 1975; Assay of deoxyribonucleic acid homology using a single-strand-specific nuclease at 75. C. J. Bacteriol 121:434–441
    [Google Scholar]
  3. Buchanan R. E., Holt J. G., Lessel E. F. Jr.ed 1966 Index Bergeyana. 1084–1085 The Williams and Wilkins Co.; Baltimore:
    [Google Scholar]
  4. Carlsson J. 1968; A numerical taxonomic study of human oral streptococci. Odontol. Revy 19:137–160
    [Google Scholar]
  5. Colman G., Williams R. E. O. 1972 Taxonomy of some human viridans streptococci. 282–299 Wannamaker L. W., Matsen J. M.ed Streptococci and streptococcal diseases Academic Press Inc.; New York:
    [Google Scholar]
  6. Coykendall A. L. 1974; Four types of Streptococcus mutans based on their genetic, antigenic and biochemical characteristics. J. Gen. Microbiol. 83:327–338
    [Google Scholar]
  7. Coykendall A. L. 1977; Proposal to elevate the subspecies of Streptococcus mutans to species status, based on their molecular composition. Int. J. Syst. Bacteriol. 27:26–30
    [Google Scholar]
  8. Coykendall A. L., Specht P. A. 1975; DNA basesequence homologies among strains of Streptococcus sanguis. J. Gen. Microbiol. 91:92–98
    [Google Scholar]
  9. Deibel R. H., Seeley H. W. Jr. 1974 Genus I. Streptococcus Rosenbach 1884, 22. 490–509 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th. The Williams and Wilkins Co.; Baltimore:
    [Google Scholar]
  10. Denhardt D. T. 1966; A membrane-filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun. 23:641–646
    [Google Scholar]
  11. Facklam R. R. 1977; Physiological differentiation of viridans streptococci. J. Clin. Microbiol. 5:184–201
    [Google Scholar]
  12. Hardie J. M., Bowden G. H. 1974 The normal microbial flora of the mouth. 47–83 Skinner F. A., Carr J. G.ed The normal microbial flora of man. Society for Applied Microbiology Symposium Series no. 3 Academic Press Inc.; London:
    [Google Scholar]
  13. London J., Chace N. M., Kline K. 1975; Aldolase of lactic acid bacteria: immunological relationships among aldolases of streptococci and gram-positive nonsporeforming anaerobes. Int. J. Syst. Bacteriol. 25:114–123
    [Google Scholar]
  14. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  15. Parker M. T., Ball L. C. 1976; Streptococci and aerococci associated with systemic infection in man. J. Med. Microbiol. 9:275–302
    [Google Scholar]
  16. Schottmuller H. 1903; Die Artunterscheidung der furden Menschen pathogenen Streptokokken durch Blutagar. Muench. Med. Wochenschr. 50:849–853
    [Google Scholar]
  17. Sherman J. M., Niven C. F. Jr., Smiley K. L. 1943; Streptococcus salivarius and other non-hemolytic streptococci of the human throat. J. Bacteriol. 45:249–263
    [Google Scholar]
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