1887

Abstract

The neutral solvent-extractable lipids and the residual bound lipids of were analyzed. The major components of the extractable lipid fraction were phosphatidylethanolamine, cardiolipin, phosphatidylglycerol, and lysocardiolipin. The high cardiolipin content was a characteristic feature. Various fatty acids were detected in the extractable lipids, the major acids being hexadecanoic and octadecenoic. The fatty acid compositions of individual phospholipids were also determined. The fatty acids of the bound lipids, which differed distinctly from those of the extractable lipids, were mainly (80%) 3-hydroxy acids (3-hydroxydecanoic acid, 3-hydroxyundecanoic acid, 3-hydroxyisoundecanoic acid, 3-hydroxydodecanoic acid, and 3-hydroxypentadecanoic acid); the remainder (20%) were saturated fatty acids including dodecanoic acid and tridecanoic acid. The amino sugar in lipid A, which was the lipid fraction of the lipopolysaccharide, was identified as glucosamine. The present findings provide additional support for the recognition of as a distinct species of the genus , even though the strains placed in this species had long been accepted as members of and produce a bacteriocin active against phase I strains of

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-28-3-394
1978-07-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/28/3/ijs-28-3-394.html?itemId=/content/journal/ijsem/10.1099/00207713-28-3-394&mimeType=html&fmt=ahah

References

  1. Abel K., de Schmertzing H., Petersen J. I. 1963; Classification of microorganisms by analysis of chemical composition. J. Bacteriol. 85:1039–1044
    [Google Scholar]
  2. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911–917
    [Google Scholar]
  3. Dawson R. M. C. 1960; A hydrolytic procedure for the identification and estimation of individual phospholipids in biological sample. Biochem. J. 75:45–53
    [Google Scholar]
  4. Hase S., Rietschel E. T. 1976; Isolation and analysis of the lipid A backbone. Lipid A structure of lipopolysaccharide from various bacterial groups. Eur. J. Biochem. 63:101–107
    [Google Scholar]
  5. Jebb W. H. H., Tomlinson A. H. 1955; The nutritional requirements of Haemophilus pertussis. J. Gen. Microbiol. 13:1–8
    [Google Scholar]
  6. Kates M. 1964; Bacterial lipids. Adv. Lipid Res. 2:17–90
    [Google Scholar]
  7. Kawai Y. 1974; Purification and characterization of per- tucin produced by Pseudomonas pertucinogena. An- timicrob. Agents Chemother. 6:347–359
    [Google Scholar]
  8. Kawai Y., Yabuuchi E. 1975; Pseudomonas pertucinogena sp. nov., an organism previously misidentified as Bordetella pertussis. Int. J. Syst. Bacteriol. 25:317–323
    [Google Scholar]
  9. Litkenhous C., Liu P. V. 1967; Bacteriocin produced by Bordetella pertussis. J. Bacteriol. 93:1484–1488
    [Google Scholar]
  10. Liideritz O., Galanos C., Lehman V., Nurminan M., Rietschel E. T., Rosenfelder G., Simon M., Westphal O. 1973; Lipid A: chemical structure and biological activity. J. Infect. Dis 128:Suppl.S17–S29
    [Google Scholar]
  11. Moss C. W-, Dees S. B. 1975; Identification of microorganisms by gas chromatographic-mass spectrométrie analysis of cellular fatty acids. J. Chromatogr. 112:595–604
    [Google Scholar]
  12. Moss C. W., Dees S. B. 1976; Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens. J. Clin. Microbiol. 4:492–502
    [Google Scholar]
  13. Moss C. W., Lambert M. W., Merwin W. H. 1974; Comparison of rapid methods for analysis of bacterial fatty acids. Appl. Microbiol. 28:80–85
    [Google Scholar]
  14. Moss C. W., Samuels S. B., Weaver R. E. 1972; Cellular fatty acid composition of selected Pseudomonas species. Appl. Microbiol. 24:596–598
    [Google Scholar]
  15. Oliver J. D., Colwell R. R. 1973; Extractable lipids of gram-negative marine bacteria: fatty acid composition. Int. J. Syst. Bacteriol. 23:442–458
    [Google Scholar]
  16. Palleroni N. J., Kunisawa R., Contopoulou R., Doudoroff M. 1973; Nucleic acid homologies in the genus Pseudomonas. Int. J. Syst. Bacteriol. 23:333–339
    [Google Scholar]
  17. Rietschel E. T., Gottert H., Liideritz O., Westphal O. 1972; Nature and linkage of the fatty acids present in the lipid A component of Salmonella lipopolysaccharides. Eur. J. Biochem. 28:166–173
    [Google Scholar]
  18. Thiele O. W., Schwinn G. 1973; The free lipids of Brucella melitensis and Bordetella pertussis. Eur. J. Biochem. 34:333–344
    [Google Scholar]
  19. Westphal O., Jann K. 1965; Bacterial lipopoly- saaccharides. Extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chem. 5:83–91
    [Google Scholar]
  20. Wilkinson S. G., Galbraith L. 1975; Studies of lipopolysaccharides from Pseudomonas aeruginosa. Eur. J. Biochem. 52:331–343
    [Google Scholar]
  21. Wilkinson S. G., Galbraith L., Lightfoot G. A. 1973; Cell walls, lipids, and lipopolysaccharides of Pseudomonas species. Eur. J. Biochem. 33:158–174
    [Google Scholar]
  22. Yano I., Ohno Y., Masui M., Kato K., Yabuuchi E., Ohyama A. 1976; Occurrence of 2- and 3-hydroxy fatty acids in high concentrations in the extractable and bound lipids of Flavobacterium meningosepticum and Flavobacterium lib. Lipids 11:685–688
    [Google Scholar]
  23. Yano I., Yabuuchi E., Shimogawa M., Ohno Y., Nasui M., Kato K. 1976; Lipids and fatty acids of Pseudomonas species and their application to chemotaxonomy. Proc. Jpn. Conf. Biochem. Lipid 18:51–54
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-28-3-394
Loading
/content/journal/ijsem/10.1099/00207713-28-3-394
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error