Intra- and Intergeneric Similarities of and Ribosomal Ribonucleic Acid Cistrons Free

Abstract

We prepared hybrids between C-labeled ribosomal ribonucleic acid (rRNA) from either NCTC 9757 or NCTC 9796 and deoxyribonucleic acid (DNA) from a great variety of named gram-negative bacteria, including many type and reference strains. Each hybrid was described by two parameters: (i) , the temperature at which 50% of the hybrid was denatured; (ii) the percent rRNA binding, the amount (micrograms) of [C]rRNA duplexed in stringent conditions per 100 μg of filter-fixed homologous or heterologous DNA. Each taxon occupied a definite area on the rRNA similarity maps. All and strains formed tight separate clusters around their neotype strains. The rRNA parameters of both taxa were about as different from each other as was the case for many genera. Both taxa are phenotypically and genotypically (by DNA-DNA hybridization) likewise very different. We proposed to elevate each cluster to genus rank as and The following strains were misnamed and belonged in neither genus: “ NCTC 10590 and 10591; “” GA; “ ATCC 17056; “” (“”) ATCC 9897, 15728, and 15729; “ (now ) NCTC 2847; and “ (now sp.) NCTC 2416. The rRNA cistrons of and resembled most closely those of section II and III, the authentic , the H-oxidizing and , and NCIB 8193. These taxa displayed a number of phenotypical similarities. We suggested that all these taxa are the closest taxonomic relatives of and

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-28-2-154
1978-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/28/2/ijs-28-2-154.html?itemId=/content/journal/ijsem/10.1099/00207713-28-2-154&mimeType=html&fmt=ahah

References

  1. Bettelheim K. A., Gordon J. F., Taylor J. 1968; The detection of a strain of Chromobacterium lividum in the tissues of certain leaf nodulated plants by the immunofluorescence technique. J. Gen. Microbiol. 54:177–184
    [Google Scholar]
  2. Breed R. S. 1957 Genus III. Chromobacterium Bergonzini, 1881. 292–296 Breed R. S., Murray E. G. D., Smith N. R.ed Bergey’s manual of determinative bacteriology, 7th. The Williams & Wilkins Co.; Baltimore, Md:
    [Google Scholar]
  3. Breed R. S., Murray E. G. D., Hitchens A. P.ed 1948 Bergey’s manual of determinative bacteriology, 6th. The Williams & Wilkins Co.; Baltimore, Md:
    [Google Scholar]
  4. Breed R. S., Murray E. G. D., Smith N. R.ed 1957 Bergey’s manual of determinative bacteriology, 7th. The Williams & Wilkins Co.; Baltimore, Md:
    [Google Scholar]
  5. Buchanan R. E., Gibbons N. E.ed 1974 Bergey’s manual of determinative bacteriology, 8th. The Williams & Wilkins Co.; Baltimore, Md:
    [Google Scholar]
  6. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62:315–323
    [Google Scholar]
  7. De Ley J. 1964; Effect of mutation on DNA-composition of some bacteria. Antonie van Leeuwenhoek J. Microbiol. Serol. 30:281–288
    [Google Scholar]
  8. De Ley J. 1968; DNA base composition of yellow Erwinia strains. Antonie van Leeuwenhoek J. Microbiol. Serol. 34:257–262
    [Google Scholar]
  9. De Ley J. 1968; DNA base composition and hybridization in the taxonomy of phytopathogenic bacteria. Annu. Rev. Phytopathol. 6:63–90
    [Google Scholar]
  10. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol. 101:738–754
    [Google Scholar]
  11. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  12. De Ley J., De Smedt J. 1975; Improvements of the membrane filter method for DNA:rRNA hybridization. Antonie van Leeuwenhoek J. Microbiol. Serol. 41:287–307
    [Google Scholar]
  13. De Ley J., Kersters K., Khan-Matsubara J., Shewan J. M. 1970; Comparative D-gluconate metabolism and DNA base composition in Achromobacter and Alcaligenes. Antonie van Leeuwenhoek J. Microbiol. Serol. 36:193–207
    [Google Scholar]
  14. De Ley J., Tytgat R. 1970; Evaluation of membrane filter methods for DNA:DNA hybridization. Antonie van Leeuwenhoek J. Microbiol. Serol. 36:461–474
    [Google Scholar]
  15. De Ley J., Van Muylem J. 1963; Some applications of deoxyribonucleic acid base composition in bacterial taxonomy. Antonie van Leeuwenhoek J. Microbiol. Serol. 29:344–358
    [Google Scholar]
  16. De Smedt J., De Ley J. 1977; Intra- and intergeneric similarities of Agrobacterium ribosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol. 27:222–240
    [Google Scholar]
  17. Doudorofif M., Palleroni N. J. 1974 Pseudomonas Migula 1894. 237217–243 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th. The Williams & Wilkins Co.; Baltimore, Md:
    [Google Scholar]
  18. Gillis M., De Ley J. 1975; Determination of the molecular complexity of double-stranded phage genome DNA from initial renaturation rates. The effect of DNA base composition. J. Mol. Biol. 98:447–464
    [Google Scholar]
  19. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem. 12:143–153
    [Google Scholar]
  20. Heberlein G. T., De Ley J., Tytgat R. 1967; Deoxyribonucleic acid homology and taxonomy of Agrobacterium, Rhizobium, and Chromobacterium. J. Bacteriol. 94:116–124
    [Google Scholar]
  21. Kirby K. S. 1957; A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein. Biochem. J. 66:495–504
    [Google Scholar]
  22. Kirby K. S., Fox-Carter E., Guest M. 1967; Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria. Biochem. J. 104:258–262
    [Google Scholar]
  23. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A.ed 1975 International code of nomenclature of bacteria. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  24. Leifson E. 1956; Morphological and physiological characteristics of the genus Chromobacterium. J. Bacteriol. 71:393–400
    [Google Scholar]
  25. Mannur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. BioL 3:208–218
    [Google Scholar]
  26. Meys W. H., Schilperoort R. A. 1971; Determination of the amount of DNA on nitrocellulose membrane filters. FEBS Lett. 12:166–168
    [Google Scholar]
  27. Moore R. L., McCarthy B. J. 1967; Comparative study of ribosomal ribonucleic acid cistrons in enterobacteria and myxobacteria. J. Bacteriol. 94:1066–1074
    [Google Scholar]
  28. Palleroni N. J., Kunisawa R., Contopoulou R., Doudoroff M. 1973; Nucleic acid homologies in the genus Pseudomonas. Int. J. Syst. Bacteriol. 23:333–339
    [Google Scholar]
  29. Sneath P. H. A. 1956; Cultural and biochemical characteristics of the genus Chromobacterium. J. Gen. Microbiol. 15:70–98
    [Google Scholar]
  30. Sneath P. H. A. 1957; The application of computers to taxonomy. J. Gen. Microbiol. 17:201–226
    [Google Scholar]
  31. Sneath P. H. A. 1960; A study of the bacterial genus Chromobacterium. Iowa State J. Sci. 34:243–500
    [Google Scholar]
  32. Sneath P. H. A. 1974 Chromobacterium Bergonzini 1881. 153354–357 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th. The Williams & Wilkins Co.; Baltimore, Md:
    [Google Scholar]
  33. Sneath P. H. A., Skerman V. B. D. 1966; A new list of type and reference strains of bacteria. Int. J. Syst. Bacteriol. 16:1–133
    [Google Scholar]
  34. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43:159–271
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-28-2-154
Loading
/content/journal/ijsem/10.1099/00207713-28-2-154
Loading

Data & Media loading...

Most cited Most Cited RSS feed