sp. nov., the Redmouth (RM) Bacterium Free

Abstract

Cultures of the redmouth (RM) bacterium, one of the etiological agents of redmouth disease in rainbow trout () and certain other fishes, were characterized by means of their biochemical reactions, by deoxyribonucleic acid (DNA) hybridization, and by determination of guanine-plus-cytosine (G+C) ratios in DNA. The DNA relatedness studies confirmed the fact that the RM bacteria are members of the family and that they comprise a single species that is not closely related to any other species of They are about 30% related to species of both and A comparison of the biochemical reactions of RM bacteria and serratiae indicated that there are many differences between these organisms and that biochemically the RM bacteria are most closely related to yersiniae. The G+C ratios of RM bacteria were approximated to be between 47.5 and 48.5%. These values are similar to those of yersiniae but markedly different from those of serratiae. On the basis of their biochemical reactions and their G+C ratios, the RM bacteria are considered to be a new species of , for which the name is proposed. Strain 2396-61 (= ATCC 29473) is designated the type strain of the species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-28-1-37
1978-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/28/1/ijs-28-1-37.html?itemId=/content/journal/ijsem/10.1099/00207713-28-1-37&mimeType=html&fmt=ahah

References

  1. Bejot J., Alonso J. M., Mollaret H. H. 1975; La diagnostic bactériologique des yersinioses humaines (infections à Yersinia pseudotuberculosis et Yersinia enterocolitica). Med. Maladies Infect. 54:233–236
    [Google Scholar]
  2. Bissett M. L. 1976; Yersinia enterocolitica isolates from humans in California, 1968-1975. J. Clin. Microbiol. 4:137–144
    [Google Scholar]
  3. Bonner T. I., Brenner D. J., Neufeld B. R., Britten R. J. 1973; Reduction in the rate of DNA reas sociation by sequence divergence. J. Mol. Biol. 81:123–135
    [Google Scholar]
  4. Brenner D. J., Falkow S. 1971; Molecular relationships among members of the Enterobacteriaceae. Adv. Genet. 16:81–118
    [Google Scholar]
  5. Brenner D. J., Fanning G. R., Rake A., Johnson K. E. 1969; A batch procedure for thermal elution of DNA from hydroxyapatite. Anal. Biochem. 28:447–459
    [Google Scholar]
  6. Brenner D. J., Fanning G. R., Skerman F. J., Falkow S. 1972; Polynucleotide sequence divergence among strains of Escherichia coli and closely related organisms. J. Bacteriol. 109:953–965
    [Google Scholar]
  7. Brenner D. J., Steigerwalt A. G., Falcao D. P., Weaver R. E., Fanning G. R. 1976; Characterization of Yersinia enterocolitica and Yersinia pseudotuberculosis by deoxyribonucleic acid hybridization and by biochemical reactions. Int. J. Syst. Bacteriol. 26:180–194
    [Google Scholar]
  8. Brenner D. J., Steigerwalt A. J., Miklus G. V., Fanning G. R. 1973; Deoxyribonucleic acid relatedness among erwiniae and other Enterobacteriaceae: the soft-rot organisms (genus Pectobacterium Waldee). Int. J. Syst. Bacteriol. 23:205–216
    [Google Scholar]
  9. Britten R. J., Kohne D. E. 1966; Nucleotide sequence repetition in DNA. 1966. Carnegie Inst. Washington Yearb. 65:78–106
    [Google Scholar]
  10. Darland G., Ewing W. H., Davis B. R. 1974 The biochemical characteristics of Yersinia enterocolitica and Yersinia pseudotuberculosis. Center for Disease Control; Atlanta, Ga:
    [Google Scholar]
  11. Edwards P. R., Ewing W. H. 1972 Identification of Enterobacteriaceae. , 3rd.. Burgess Publishing Co.; Minneapolis Minn:
    [Google Scholar]
  12. Ewing W. H., Davis B. R. 1970 Media and tests for differentiation of Enterobacteriaceae. Center for Disease Control; Atlanta, Ga:
    [Google Scholar]
  13. Ewing W. H., Davis B. R. 1972; Biochemical characterization of Serratia marcescens. Public Health Lab. 30:211–226
    [Google Scholar]
  14. Ewing W. H., Davis B.R., Fife M. A., Lessel E. F. 1973; Biochemical characterization of Serratia liquefaciens (Grimes and Hennerty) Bascomb et al. (formerly Enterobacter liquefaciens) and Serratia rub- idaea (Stapp) comb. nov. and designation of neotype strains. Int. J. Syst. Bacteriol. 23:217–225
    [Google Scholar]
  15. Mäkelä P. H., Mayer H. 1976; Enterobacterial common antigen. Bacteriol. Rev. 40:519–632
    [Google Scholar]
  16. Mollaret H. H., Thai E. 1974 Genus XI. Yersinia van Loghem 1944, 15. 330–332 Buchanan R. E., Gibbons N. E.ed Bergey’s manual of determinative bacteriology, 8th. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  17. Ross A. J., Rucker R. R., Ewing W. H. 1966; Description of a bacterium associated with redmouth disease of rainbow trout (Salmo gairdneri). Can. J. Microbiol. 12:763–770
    [Google Scholar]
  18. Rucker R. R. 1966; Redmouth disease in rainbow trout (Salmo gairdneri). Bull. Off. Int. Epiz. 65:825–830
    [Google Scholar]
  19. Steigerwalt A. G., Fanning G. R., Fife-Asbury M. A., Brenner D. J. 1976; DNA relatedness among species of Enterobacter and Serratia. Can. J. Microbiol. 22:121–137
    [Google Scholar]
  20. Wobeser G. 1973; An outbreak of redmouth disease in rainbow trout (Salmo gairdneri) in Saskatchewan. J. Fish. Res. Board Can. 30:571–575
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-28-1-37
Loading
/content/journal/ijsem/10.1099/00207713-28-1-37
Loading

Data & Media loading...

Most cited Most Cited RSS feed