1887

Abstract

A survey of 272 free-living rhizobia strains showed that a majority of the and unclassified isolates are capable of both growth and acetylene reduction on a Casamino Acids-minimal medium. Two other taxonomic categories of were discernible: and , which grow but do not reduce acetylene; and , which fails to grow on the minimal medium. Sixty-nine strains were acetylene reducers obtained from root nodules of the plant hosts: (partridge pea), (wild indigo), (thornbroom), (Florida beggarweed), (peanut), (velvet bean), (kudzu), (cowpea), , and (soybean). The extent of acetylene reduction varied depending on both the strain and O tension, suggesting that reduction activity differs according to the cellular organization of individual strains.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-28-1-27
1978-01-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/28/1/ijs-28-1-27.html?itemId=/content/journal/ijsem/10.1099/00207713-28-1-27&mimeType=html&fmt=ahah

References

  1. Allen O. N., Hamatova E., Skinner F. A. 1973 IBP world catalogue of Rhizobium collections. International Biological Programme Section PP. Knapp Drewett & Sons, Ltd.; Kingston-upon-Thames, England:
    [Google Scholar]
  2. Anderson S. J., Phillips D. A. 1976; Effect of protein additives on acetylene reduction (nitrogen fixation) by Rhizobium in the presence and absence of soybean cells. Plant Physiol. 57:890–893
    [Google Scholar]
  3. Burris R. H. 1974 Methodology. 9–33 Quispel A.ed The biology of nitrogen fixation North-Holland Publishing Co.; Amsterdam:
    [Google Scholar]
  4. De Ley J., Rassel A. 1965; DNA base composition, flagellation and taxonomy of the genus Rhizobium. J. Gen. Microbiol. 41:85–91
    [Google Scholar]
  5. Eady R. R., Postgate J. R. 1974; Nitrogenase. Nature (London) 249:805–810
    [Google Scholar]
  6. Evans W. R., Keister D. L. 1976; Reduction of acetylene by stationary cultures of free-living Rhizobium sp. under atmospheric oxygen levels. Can. J. Microbiol. 22:949–952
    [Google Scholar]
  7. Graham P. M. 1963; Vitamin requirements of root nodule bacteria. J. Gen. Microbiol. 30:245–248
    [Google Scholar]
  8. Hardy R. W. F., Holsten R. D., Jackson E. K., Burns R. C. 1968; The acetylene-ethylene assay for N-2 fixation: laboratory and field evaluation. Plant Physiol. 43:1185–1207
    [Google Scholar]
  9. Heberlein G. T., De Ley J., Tijtgat R. 1967; Deoxyribonucleic acid homology and taxonomy of Agrobacterium, Rhizobium, and Chromobacterium. J. Bacteriol. 94:116–124
    [Google Scholar]
  10. Heywood W. H. 1971 The Leguminosae—a systematic purview. 1–29 Harbome J. B., Boulter D., Turner B. L.ed Chemotaxonomy of the Leguminosae Academic Press Inc.; New York:
    [Google Scholar]
  11. Keister D. L. 1975; Acetylene reduction by pure cultures of rhizobia. J. Bacteriol. 123:1265–1268
    [Google Scholar]
  12. Keister D. L., Evans W. R. 1976; Oxygen requirement for acetylene reduction by pure cultures of rhizobia. J. Bacteriol. 129:149–153
    [Google Scholar]
  13. Kurz W. G. W., La Rue T. A. 1975; Nitrogenase activity in rhizobia in absence of plant host. Nature (London) 256:407–409
    [Google Scholar]
  14. Layne E. 1957; Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol. 3:448–450
    [Google Scholar]
  15. McComb J. A., Elliott J., Dilworth M. J. 1975; Acetylene reduction by Rhizobium in pure culture. Nature (London) 256:409–410
    [Google Scholar]
  16. Oppenheim J., Fisher R. J., Wilson P. W., Marcus L. 1970; Properties of a soluble nitrogenase in Azotobacter. J. Bacteriol. 101:292–296
    [Google Scholar]
  17. Pagan J. D., Child J. J., Scowcroft W. R., Gibson A. H. 1975; Nitrogen fixation by Rhizobium cultured on a defined medium. Nature (London) 256:406–407
    [Google Scholar]
  18. The American Type Culture Collection 1974 Medium 111. 274 Catalogue of strains American Type Culture Collection; Rockville, Md:
    [Google Scholar]
  19. Tjepkema J., Evans H. J. 1975; Nitrogen fixation by free-living Rhizobium in a defined liquid medium. Biochem. Biophys. Res. Commun. 65:625–628
    [Google Scholar]
  20. Vincent J. M. 1974 Root-nodule symbioses with Rhizobium. 266–341 Quispel A.ed The biology of nitrogen fixation North-Holland Publishing Co.; Amsterdam:
    [Google Scholar]
  21. Werner D., Berghauser K. 1976; Discrimination of Rhizobium japonicum, Rhizobium lupini, Rhizobium trifolii, Rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate. Arch. Microbiol. 107:257–262
    [Google Scholar]
  22. Yates M. G., Jones C. W. 1974; Respiration and nitrogen fixation in Azotobacter. Adv. Microb. Physiol. 11:97–135
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-28-1-27
Loading
/content/journal/ijsem/10.1099/00207713-28-1-27
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error