A survey of 272 free-living rhizobia strains showed that a majority of the and unclassified isolates are capable of both growth and acetylene reduction on a Casamino Acids-minimal medium. Two other taxonomic categories of were discernible: and , which grow but do not reduce acetylene; and , which fails to grow on the minimal medium. Sixty-nine strains were acetylene reducers obtained from root nodules of the plant hosts: (partridge pea), (wild indigo), (thornbroom), (Florida beggarweed), (peanut), (velvet bean), (kudzu), (cowpea), , and (soybean). The extent of acetylene reduction varied depending on both the strain and O tension, suggesting that reduction activity differs according to the cellular organization of individual strains.


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