Ten obligately anaerobic mycoplasma strains isolated from the rumen of sheep and cattle were grouped serologically by agglutination and gel diffusion precipitin tests. They could be placed into four serovars that fit well with group separations based upon cultural, biochemical, and biophysical properties. Hyperimmune sera prepared against a strain representing each serovar showed no cross-species interaction when tested with antigens of several known aerobic bovine mycoplasma and acholeplasma strains. Growth inhibition tests of strains confirmed the grouping by agglutination and gel diffusion precipitin tests.


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