Discontinuous electrophoresis techniques were used to analyze 161 isolates from Asia, Southeast Asia, Java, Madagascar, Africa, and North and South America. The application of similarity matrix analysis to electrophophoretic data from isolates of each geographic region led to the grouping of the isolates into 57 electropherotypes which, in turn, form 11 electropherotype groups representing major protein variants. Of the 161 isolates tested, only seven, belonging to two pairs of the 57 electropherotypes, could not be differentiated using the electrophoretic analysis and six biochemical tests. In addition, the distribution of certain of the 11 major electropherotype groups corresponds with known routes of dispersion of Y. during the present pandemic.


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