@article{mbs:/content/journal/ijsem/10.1099/00207713-25-3-304, author = "Fukazawa, Y. and Nakase, T. and Shinoda, T. and Nishikawa, A. and Kagaya, K. and Tsuchiya, T.", title = "Significance of Cell Wall Structures on Yeast Classification: Proton Magnetic Resonance and Serological and Deoxyribonucleic Acid Characterization of Candida sake and Related Species", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1975", volume = "25", number = "3", pages = "304-314", doi = "https://doi.org/10.1099/00207713-25-3-304", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-25-3-304", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "The serological properties and patterns of proton magnetic resonance spectra of cell wall mannans were characterized in 15 strains of Candida sake including the previous taxa C. tropicalis var. lambica, C. maltosa, C. vanriji, C. salmonicola, C. cloacae, C. parapsilosis var. intermedia, and C. natalensis. The proton magnetic resonance spectra of the mannans were divided into four patternographic types. Serological analyses also demonstrated a division into four serotypes that corresponded to the four proton magnetic resonance spectral types. Antigenic patterns of groups I and II closely resembled those of C. tropicalis and C. subtropicalis, respectively; strains of group III were related to both C. parapsilosis and S. cerevisiae; and strains of group IV were closely related to S. cerevisiae. The guanine plus cytosine contents of the deoxyribonucleic acid of group I and group II strains were heterogeneous, whereas strains of groups III and IV showed guanine plus cytosine values with narrow ranges within each group. These data suggest that the four groupings represent at least four species.", }