1887

Abstract

Obligately anaerobic, filterable microorganisms have been isolated from the rumens of cattle and sheep. On the basis of typical colonial appearance, lack of cell wall, filterability through a 450-nm membrane filter, absence of reversion to a bacterial form, and inhibition of growth by homologous antiserum, these organisms fit the description for organisms in the order They exhibit unique cultural, biochemical, and serological properties, and we have thus placed them in a new genus, is the type species of the new genus. Strain 6-1, a sterol-requiring strain, has been designated as the type strain of and has been deposited in the American Type Culture Collection as ATCC 27879; a non-sterol-requiring strain, number 161, has been deposited as ATCC 27880. The relationship of to , an anaerobic mycoplasma possessing extracellular proteolytic and bactoclastic enzymes, is discussed.

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/content/journal/ijsem/10.1099/00207713-25-2-173
1975-04-01
2022-09-29
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References

  1. Allison M. J., Bucklin J. A., Dougherty R. W. 1964; Ruminal changes after overfeeding with wheat and the effect of intraruminal inoculation on adaptation to a ration containing wheat. J. Anim. Sci 23:1164–1171
    [Google Scholar]
  2. Barker S. B., Summerson W. H. 1941; The colori- meteric determination of lactic acid in biological materials. J. Biol. Chem 138:535–554
    [Google Scholar]
  3. Boivin A., Mesrobeanu I., Mesrobeanu L. 1933; Technique pour la preparation des polysaccharides microbiens specifiques. C. R. Soc. Biol 113:490–492
    [Google Scholar]
  4. Boothe A. D., Van Der Maaten M. J., Malmquist W. A. 1970; Morphological variation of a syncytial virus from lymphosarcomatous and apparently normal cattle. Arch. Gesamte Virusforsch 31:373–384
    [Google Scholar]
  5. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochim. Biophys. Acta 34:103–110
    [Google Scholar]
  6. Bryant M. P., Burkey L. A. 1953; Cultural methods and some characteristics of some of the numerous groups of bacteria in the bovine rumen. J. Dairy Sci 36:205–217
    [Google Scholar]
  7. Bryant M. P., Robinson I. M. 1961; An improved nonselective culture medium for ruminal bacteria and its use in determining diurnal variation in numbers of bacteria in the rumen. J. Dairy Sci 44:1446–1456
    [Google Scholar]
  8. Bryant M. P., Robinson I. M. 1962; Some nutri tional characteristics of predominant culturable ruminal bacteria. J. Bacteriol 84:605–614
    [Google Scholar]
  9. Burton K. 1968; Determination of DNA concentration with diphenylamine. p 163–166 In Grossman L., Moldave K. ed Methods in enzymology, vol. 12. Academic Press Inc; New York:
    [Google Scholar]
  10. Caldwell D. R., Bryant M. P. 1966; Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl. Microbiol 14:794–801
    [Google Scholar]
  11. Hayes F. N. 1963; Solutes and solvents for liquid scintillation counting. Packard technical bulletin no. 1. Packard Instrument Co., Inc; Downers Grove:I11
    [Google Scholar]
  12. Holdeman L. V., Moore W. E. C. ed 1972; Anaerobic laboratory manual. Virginia Polytechnic Institute and State University; Blacksburg, Va:
    [Google Scholar]
  13. Hungate R. E. 1966; The rumen and its microbes. Academic Press Inc; New York:
    [Google Scholar]
  14. McDonald J. A., Speeg K. V. Jr, Campbell J. W. 1972; Urease: a sensitive and specific radiometric assay. Enzymologia 42:1–9
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyri bonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  16. Miura K. 1967; Preparation of bacterial DNA by the phenol-pH-9-RNases method. p 543–545 In Grossman L., Moldave K. ed Methods in enzymology, vol. 12. Academic Press Inc; New York:
    [Google Scholar]
  17. Razin S., Shafer Z. 1969; Incorporation of choles terol by membranes of bacterial L-phase variants. J. Gen. Microbiol 58:327–339
    [Google Scholar]
  18. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol 102:306–310
    [Google Scholar]
  19. Ritchie A. E., Femelius A. O. 1969; Characteriza tion of bovine viral diarrhea viruses. Arch. Gesamte Virusforsch 5:369–389
    [Google Scholar]
  20. Robinson I. M., Allison M. J. 1975; Transfer of Acholeplasma bactoclasticum Robinson and Hungate to the genus Anaeroplasma (Anaeroplasma bacto-clasticum [Robinson and Hungate] comb.nov.): emended description of the species. Int. J. Syst. Bacteriol 25:182–186
    [Google Scholar]
  21. Robinson J. P., Hungate R. E. 1973; Acholeplasma bactoclasticum sp. n., an anaerobic mycoplasma from the bovine rumen. Int. J. Syst. Bacteriol 23:171–181
    [Google Scholar]
  22. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol 4:430–443
    [Google Scholar]
  23. Schubert K., Rose G., Horhold C. 1969; Sterols in bacteria. p 53–56 In Schubert K. ed Symposium über biochemische aspekte der steriodforschung. Akademie-Verlag; Berlin:
    [Google Scholar]
  24. Simmons D. G., Lukert P. D. 1972; Isolation of an anaerobic mycoplasma from avian cell cultures and some of its effects of Marek’s disease virus. Avian Dis 16:521–528
    [Google Scholar]
  25. Smith P. F., Rothblat G. H. 1960; Incorporation of cholesterol by pleuropneumonia-like organisms. J. Bacteriol 80:842–850
    [Google Scholar]
  26. Subcomittee on the Taxonomy of Mycoplasmatales 1972; Proposal for minimal standards for descriptions of new species of the order Mycoplasmatales. Int. J. Syst. Bacteriol 22184–188
    [Google Scholar]
  27. Vandemark P. J. 1969; Respiratory pathways in the Mycoplasma,. p 491–501 In Hayflick L. ed The Mycoplasmatales and the L-phase of bacteria. Apple- ton-Century-Crofts; New York:
    [Google Scholar]
  28. Wainer A. 1967; The chromatography of ninhydrin negative compounds on an amino acid analyzer column. J. Chromatogr 26:48–53
    [Google Scholar]
  29. Ward J. R., Martin C. H. 1962; Production of L-phase variants of bacteria with cycloserine. Proc. Soc. Exp. Biol. Med 111:156–160
    [Google Scholar]
  30. Westphal O., Luderitz O., Bister F. 1952; Extrac tion of bacteria with phenol water. Z. Naturforsch 7B:149–155
    [Google Scholar]
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