D-Lactate dehydrogenase (D-LDH) and glucose-6-phosphate dehydrogenase (G-6-P-DH), both purified from strain NCDO 546, were used to prepare specific antisera. Cross-reactions of varying intensity with the anti-D-LDH were obtained with all of the strains and with certain strains of the heterofermentative lactobacilli studied. Cross-reactions with the anti-G-6-P-DH were obtained with all of the strains except those of ; none of the heterofermentative lactobacilli cross-reacted. Pairwise comparisons between cross-reacting extracts with respect to each antiserum permitted the assignment of strains to groups of identical immunological specificity: with the exception of a single strain, the groups of strains revealed by the use of each antiserum coincided.


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