RT Journal Article SR Electronic(1) A1 Pauley, E. H. A1 Krieg, N. R.YR 1974 T1 Long-Term Preservation of Spirillum volutans JF International Journal of Systematic and Evolutionary Microbiology, VO 24 IS 2 SP 292 OP 293 DO https://doi.org/10.1099/00207713-24-2-292 PB Microbiology Society, SN 1466-5034, AB Strains of Spirillum volutans, the type species of the genus Spirillum, have not been satisfactorily preserved in the past except by serial transfer. Long-term preservation of such strains can be achieved in liquid nitrogen by the use of dimethyl sulfoxide and a low-salt suspending medium. Glycerol is not satisfactory as a cryoprotective agent., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-24-2-292