@article{mbs:/content/journal/ijsem/10.1099/00207713-24-2-292, author = "Pauley, E. H. and Krieg, N. R.", title = "Long-Term Preservation of Spirillum volutans", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1974", volume = "24", number = "2", pages = "292-293", doi = "https://doi.org/10.1099/00207713-24-2-292", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-24-2-292", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Strains of Spirillum volutans, the type species of the genus Spirillum, have not been satisfactorily preserved in the past except by serial transfer. Long-term preservation of such strains can be achieved in liquid nitrogen by the use of dimethyl sulfoxide and a low-salt suspending medium. Glycerol is not satisfactory as a cryoprotective agent.", }