The mole percent guanine plus cytosine in the deoxyribonucleic acid of 12 strains (6 species) of Leuconostoc and 9 strains (4 species) of slime-forming heterofermentative lactobacilli has been determined. Calculated from melting-temperature curves, the values were in the range 40 to 47%, but calculated from buoyant-density determinations they were 2 to 4% lower. The melting temperature of the reference deoxyribonucleic acid from Escherichia coli K-12 was 0.7 to 1 degree higher than the expected figure of 90.6 C. This point is discussed.
BrutonK.1968; Determination of DNA concentration with diphenylamine. p. 163–165 In
GrossmanL.,
MaldareK.
(ed.) Methods in enzymology, vol. 12 B. Academic PressInc; New York:
de LeyJ.1969; Re-examination of the association between melting point, buoyant density and chemical base composition of deoxyribonucleic acid. J. Bacteriol101738–754
MandelM.,
RowndR.1964; Deoxyribonucleic acid base composition in the Enterobacteriaceae,an evolutionary sequence?. p. 585–597 In
LeoneC. A.
(ed.) Taxonomic biochemistry and serology. Roland Press & Co; New York:
SchildkrautC. L.,
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCI. J. Mol. Biol4430–443