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The mole percent guanine plus cytosine in the deoxyribonucleic acid of 12 strains (6 species) of Leuconostoc and 9 strains (4 species) of slime-forming heterofermentative lactobacilli has been determined. Calculated from melting-temperature curves, the values were in the range 40 to 47%, but calculated from buoyant-density determinations they were 2 to 4% lower. The melting temperature of the reference deoxyribonucleic acid from Escherichia coli K-12 was 0.7 to 1 degree higher than the expected figure of 90.6 C. This point is discussed.