1887

Abstract

Bacteriophages for the genus were isolated from sewage samples. On the basis of plaque morphology and host specificity, three different phage isolates were obtained and purified by standard techniques. Cross-neutralization experiments with homologous and heterologous phage antisera showed no serological relatedness among the three phages and, therefore, they were placed into three serological groups. Electron micrographs showed the isolates to have different and distinctive morphological types, one of which was unique in that it possessed an elongated "bullet-shaped" capsid. Nucleic acid types for the three phages were determined by the acridine orange-fluorescent staining technique to be double-stranded deoxyribonucleic acid. Based on serological grouping, the three phages were classified and designated DM-11, DM-21, and DM-31. The value of the phage isolates in defining the species of the genus is discussed.

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/content/journal/ijsem/10.1099/00207713-24-2-230
1974-04-01
2022-01-20
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References

  1. Adams M.H. 1959; Bacteriophages. Interscience Publishers, Inc; New York:
    [Google Scholar]
  2. Agabian-Keshian N., Shapiro L. 1970; Stalked bacteria: properties of deoxyribonucleic acid bacteriophage CbK. J. Virol 5:795–800
    [Google Scholar]
  3. Bradley D.E. 1966; The fluorescent staining of bacteriophage nucleic acids. J. Gen. Microbiol 44:383–391
    [Google Scholar]
  4. Bradley D.E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriol. Rev 31:230–314
    [Google Scholar]
  5. Ewing W.H., Davis B.R. 1972; Biochemical characterization of Citrobacter diversus (Burkey) Werkman and Gillen and designation of the neotype strain. Int. J. Syst. Bacteriol 22:12–18
    [Google Scholar]
  6. Werkman C. H., Gillen G.F. 1932; Bacteria producing trimethylene glycol. J. Bacteriol 23:167–182
    [Google Scholar]
  7. Young V.M., Kenton D.M., Hobbs B.S., Moody M.R. 1974; Levinea, a new genus of the family Enterobacteriaceae. Int. J. Syst. Bacteriol 21:58–63
    [Google Scholar]
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