RT Journal Article SR Electronic(1) A1 Schaad, Norman W.YR 1974 T1 Comparative Immunology of Ribosomes and Disc Gel Electrophoresis of Ribosomal Proteins from Erwiniae, Pectobacteria, and Other Members of the Family Enterobacteriaceae JF International Journal of Systematic and Evolutionary Microbiology, VO 24 IS 1 SP 42 OP 53 DO https://doi.org/10.1099/00207713-24-1-42 PB Microbiology Society, SN 1466-5034, AB Ribosomes from erwiniae, pectobacteria, and other Enterobacteriaceae were compared by two criteria: (i) disc gel electrophoresis of 50S subunit proteins, and (ii) Ouchterlony gel diffusion of intact ribosomes. There were no significant differences in the electrophoretic protein profiles of strains of Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, or Enterobacter agglomerans (synonym: Erwinia herbicola). The only signficant profile differences occurred with the faster-migrating proteins of enterobacteria grouped according to plant or animal source. Several non-enterobacteria also had similar over-all profiles; however, specific differences were evident. Immuno-diffusion data were effective in establishing relatedness at the subspecies level. A single antigenic determinant was responsible for the specificity and was found to differ in its sensitivity to treatment with trypsin, ribonuclease, or heat., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-24-1-42