%0 Journal Article %A OLIVER, James D. %A COLWELL, Rita R. %T Extractable Lipids of Gram-Negative Marine Bacteria: Fatty-Acid Composition %D 1973 %J International Journal of Systematic and Evolutionary Microbiology, %V 23 %N 4 %P 442-458 %@ 1466-5034 %R https://doi.org/10.1099/00207713-23-4-442 %I Microbiology Society, %X Fatty-acid compositions were determined for 20 strains of marine and estuarine bacteria and two strains representative of terrestrial species. Results showed that the fatty acids of marine bacteria differed little from those of nonmarine organisms, and a primary role for hexadecenoic acid was indicated. Of the 20 strains examined, with the exception of one, the major fatty-acid species were C16, C16:1, and C 18:1. Significant differences were observed among the fatty-acid patterns of the various bacterial genera included in the set of 20 strains examined, and rapid differentiation of most of the genera could thus be accomplished. A recently isolated marine species demonstrated a unique fatty-acid pattern wherein branched acids formed the major fatty-acid class. Effects of culture age, growth temperature, and salt concentration of the medium on the fatty-acid profiles were also investigated. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-23-4-442