@article{mbs:/content/journal/ijsem/10.1099/00207713-23-3-238, author = "BOYER, E. W. and INGLE, M. B. and MERCER, G. D.", title = "Bacillus alcalophilus subsp. halodurans subsp. nov.: An Alkaline-Amylase-Producing, Alkalophilic Organism", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1973", volume = "23", number = "3", pages = "238-242", doi = "https://doi.org/10.1099/00207713-23-3-238", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-23-3-238", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "An alkaline-amylase-producing, alkalophilic bacillus, NRRL B-3881, was characterized and compared with Bacillus sp. ATCC 21591 and Bacillus alcalophilus Vedder strain NCTC 4553 (=ATCC 27647), which is here designated as the type strain of B. alcalophilus. All three strains contained. motile, gram-positive rods with rounded ends and swollen, clavate sporangia with oval, terminal to subterminal endospores. All three strains grew in soybean broth; were facultatively anaerobic; hydrolyzed starch, gelatin, and casein; reduced methylene blue; and fermented the following carbohydrates without gas production: sucrose, D-glucose, lactose, maltose, D-mannitol, D-xylose, L-arabinose, glycerol, sorbitol, and salicin. None produced acetylmethylcarbinol, indole, urease, or crystalline dextrins. Bacillus sp. ATCC 21591 and Bacillus sp. NRRL B-3881, but not NCTC 4553, reduced nitrate to nitrite, utilized citrate, and grew well in 12% NaCl and slowly in 15% NaCl. B. alcalophilus NCTC 4553 did not grow in 5% NaCl. In our opinion, these differences are sufficient to justify the establishment of a separate subspecies for ATCC 21591, NRRL B-3881, and similar strains. We propose the name B. alcalophilus subsp. halodurans as the name for this new subspecies. The name of the type subspecies, which contains the type strain, NCTC 4553, is B. alcalophilus subsp. alcalophilus Vedder. NRRL B-3881 is designated as the type strain of B. alcalophilus subsp. halodurans and is available from the Northern Regional Research Laboratory. It has also been deposited in the American Type Culture Collection under the number 27557.", }