1887

Abstract

An alkaline-amylase-producing, alkalophilic bacillus, NRRL B-3881, was characterized and compared with sp. ATCC 21591 and Vedder strain NCTC 4553 (=ATCC 27647), which is here designated as the type strain of . . All three strains contained. motile, gram-positive rods with rounded ends and swollen, clavate sporangia with oval, terminal to subterminal endospores. All three strains grew in soybean broth; were facultatively anaerobic; hydrolyzed starch, gelatin, and casein; reduced methylene blue; and fermented the following carbohydrates without gas production: sucrose, D-glucose, lactose, maltose, D-mannitol, D-xylose, L-arabinose, glycerol, sorbitol, and salicin. None produced acetylmethylcarbinol, indole, urease, or crystalline dextrins. sp. ATCC 21591 and sp. NRRL B-3881, but not NCTC 4553, reduced nitrate to nitrite, utilized citrate, and grew well in 12% NaCl and slowly in 15% NaCl. . NCTC 4553 did not grow in 5% NaCl. In our opinion, these differences are sufficient to justify the establishment of a separate subspecies for ATCC 21591, NRRL B-3881, and similar strains. We propose the name . subsp. as the name for this new subspecies. The name of the type subspecies, which contains the type strain, NCTC 4553, is . subsp. Vedder. NRRL B-3881 is designated as the type strain of . subsp. and is available from the Northern Regional Research Laboratory. It has also been deposited in the American Type Culture Collection under the number 27557.

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/content/journal/ijsem/10.1099/00207713-23-3-238
1973-07-01
2022-08-13
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