1887

Abstract

An alkaline-amylase-producing, alkalophilic bacillus, NRRL B-3881, was characterized and compared with sp. ATCC 21591 and Vedder strain NCTC 4553 (=ATCC 27647), which is here designated as the type strain of . . All three strains contained. motile, gram-positive rods with rounded ends and swollen, clavate sporangia with oval, terminal to subterminal endospores. All three strains grew in soybean broth; were facultatively anaerobic; hydrolyzed starch, gelatin, and casein; reduced methylene blue; and fermented the following carbohydrates without gas production: sucrose, D-glucose, lactose, maltose, D-mannitol, D-xylose, L-arabinose, glycerol, sorbitol, and salicin. None produced acetylmethylcarbinol, indole, urease, or crystalline dextrins. sp. ATCC 21591 and sp. NRRL B-3881, but not NCTC 4553, reduced nitrate to nitrite, utilized citrate, and grew well in 12% NaCl and slowly in 15% NaCl. . NCTC 4553 did not grow in 5% NaCl. In our opinion, these differences are sufficient to justify the establishment of a separate subspecies for ATCC 21591, NRRL B-3881, and similar strains. We propose the name . subsp. as the name for this new subspecies. The name of the type subspecies, which contains the type strain, NCTC 4553, is . subsp. Vedder. NRRL B-3881 is designated as the type strain of . subsp. and is available from the Northern Regional Research Laboratory. It has also been deposited in the American Type Culture Collection under the number 27557.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-23-3-238
1973-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/23/3/ijs-23-3-238.html?itemId=/content/journal/ijsem/10.1099/00207713-23-3-238&mimeType=html&fmt=ahah

References

  1. Boyer E. W., Ingle M. B. 1972; Extracellular alkaline amylase from a Bacillus species. J. Bacteriol 110:992–1000
    [Google Scholar]
  2. Buchanan R. E., Holt J. G., Lessel E. F. Jr 1966 Index Bergeyana The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  3. Chislett M. E., Kushner D. J. 1961; A strain of Bacillus circulans capable of growing under highly alkaline conditions. J. Gen. Microbiol 24:187–190
    [Google Scholar]
  4. Horikoshi K. 1971; Production of alkaline enzymes by alkalophilic microorganisms. Part I. Alkaline protease produced by Bacillus no. 221. Agr. Biol. Chem. 35:1407–1414
    [Google Scholar]
  5. Horikoshi K. 1971; Production of alkaline enzymes by alkalophilic microorganisms. Part II. Alkaline amylase produced by Bacillus no. A-40-2. Agr. Biol. Chem 35:1783–1791
    [Google Scholar]
  6. Horikoshi K. 1972; Production of alkaline enzymes by alkalophilic microorganisms. III. Alkaline pectinase of Bacillus no. P-4-N. Agr. Biol. Chem 36:285–293
    [Google Scholar]
  7. Ingle M. B., Boyer E. W. 1972; Alkaline amylase synthesis by a Bacillus species. Develop. Ind. Microbiol 13:421–427
    [Google Scholar]
  8. Smith N. R. 1948; Genus I. Bacillus Cohn. 705–762 Breed R. S., Murray E. G. D., Hitchens A. P. Bergey’s manual of determinative bacteriology, 6. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  9. Smith N. R., Gordon R. E. 1957; Genus I. Bacillus Cohn. 613–634 Breed R. S., Murray E. G. D., Smith N. R. Bergey’s manual of determinative bacteriology, 7. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  10. Smith N. R., Gordon R. E., Clark F. E. 1952; Aerobic sporeforming bacteria. U.S. Dept. Agr. Monogr16 Washington, D.C.:
    [Google Scholar]
  11. Vedder A. 1934; Bacillus alcalophilus n. sp.; benevens enkle ervaringen met sterk alcalische voedingsbodems. Antonie van Leeuwenhoek J. Microbiol. Serol 1:141–147
    [Google Scholar]
  12. Yamamoto M., Tanaka Y., Horikoshi K. 1972; Alkaline amylases of alkalophilic bacteria. Agr. Biol. Chem 36:1819–1823
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-23-3-238
Loading
/content/journal/ijsem/10.1099/00207713-23-3-238
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error