An alkaline-amylase-producing, alkalophilic bacillus, NRRL B-3881, was characterized and compared with sp. ATCC 21591 and Vedder strain NCTC 4553 (=ATCC 27647), which is here designated as the type strain of All three strains contained motile, gram-positive rods with rounded ends and swollen, clavate sporangia with oval, terminal to subterminal endospores. All three strains grew in soybean broth; were facultatively anaerobic; hydrolyzed starch, gelatin, and casein; reduced methylene blue; and fermented the following carbohydrates without gas production: Sucrose, D-glucose, lactose, maltose, D-mannitol, D-xylose, L-arabinose, glycerol, sorbitol, and salicin. None produced acetylmethylcarbinol, indole, unrease, or crystalline dextrins. sp. ATCC 21591 and sp. NRRL B-3881, but not NCTC 4553, reduced nitrate to nitrite, utilized citrate, and grew well in 12% NaCl and slowly in 15% NaCl. NCTC 4553 did not grow in 5% NaCl. In our opinion, these differences are sufficient to justify the establishment of a separate subspecies for ATCC 21591, NRRL B-3881, and similar strains. We propose the name subsp. as the name for this new subspecies. The name of the type subspecies, which contains the type strain, NCTC 4553, is subsp. Vedder. NRRL B-3881 is designated as the type strain of subsp. and is available from the Northern Regional Research Laboratory. It has also been deposited in the American Type Culture Collection under the number 27557.


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