1887

Abstract

Genetic relatedness among 51 strains of fluorescent pseudomonads comprising 18 plant pathogenic species and two saprophytic species were determined by in vitro nucleic acid hybridization. Four of six homologous groups, shown to be distinct genotypically, are provisionally designated as: “syringae,” “morsprunorum,” “viridiflava,” and “cichorii.” Two other homology groups seemed to be distinct, but because of lack of tests with reference strains the amount of intragroup relatedness is unknown. Thus, these somewhat artificial groups are provisionally designated “tomato” and “marginalis.”

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1973-04-01
2022-01-25
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References

  1. Billing E. 1970; Further studies on the phage sensitivity and the determination of phytopathogenic Pseudomonas spp. J. Appl. Bacteriol 33:478–491
    [Google Scholar]
  2. Billing E. 1970; Pseudomonas viridiflava (Burkholder, 1930; Clara, 1934). J. Appl. Bacteriol 33:492–500
    [Google Scholar]
  3. Burton K. 1967; Determination of DNA concentration with diphenylamine. 163–166 Grossman L., Moldave K. Methods in enzymology 12 part B Academic Press Inc.; New York:
    [Google Scholar]
  4. DeLey J. 1968; DNA base composition and hybridization in the taxonomy of phytopathogenic bacteria. Annu. Rev. Phytopathol 6:63–90
    [Google Scholar]
  5. DeLey J., Tijtgat R. 1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek. J. Microbiol. Serol 36:461–474
    [Google Scholar]
  6. Garrett C. M. E., Panagopoulos C. G., Crosse J. E. 1966; Comparison of plant pathogenic pseudomonads from fruit trees. J. Appl. Bacteriol 29:342–355
    [Google Scholar]
  7. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. Mol. Biol 12:829–842
    [Google Scholar]
  8. Jackson J. F., Sands D. C. 1970; DNA base composition of some phytopathogenic pseudomonads. Phytopathology 60:1863–1864
    [Google Scholar]
  9. Johnson J. L., Ordal E. J. 1968; Deoxyribonucleic acid homology in bacterial taxonomy: effect of incubation temperature on reaction specificity. J. Bacteriol 95:893–900
    [Google Scholar]
  10. Kado C. I., Heskett M. G. 1970; Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas, and Xanthomonas . Phytopathology 60:969–976
    [Google Scholar]
  11. Lelliott R. A., Billing E., Hayward A. C. 1966; A determinative scheme for the fluorescent plant pathogenic pseudomonads. J. Appl. Bacteriol 29:470–489
    [Google Scholar]
  12. Lovrekovich L., Klement Z., Dowson W. J. 1963; Serological investigation of Pseudomonas syringae and Pseudomonas morsprunorum strains. Phytopathol. Z 47:19–24
    [Google Scholar]
  13. McCarthy B. J., Bolton E. T. 1963; An approach to the measurement of genetic relatedness among organisms. Proc. Nat. Acad. SciU.S. A 50:156–164
    [Google Scholar]
  14. Mandel M. 1966; Deoxyribonucleic acid base composition in the genus Pseudomonas . J. Gen. Microbiol 43:273–292
    [Google Scholar]
  15. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. 195–206 Grossman L., Moldave K. Methods in enzymology 12 part B Academic Press Inc; New York:
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  17. Misaghi I., Grogan R. G. 1969; Nutritional and biochemical comparisons of plant-pathogenic and saprophytic fluorescent pseudomonads. Phytopathology 59:1436–1450
    [Google Scholar]
  18. Miura K. 1967; Preparation of bacterial DNA by the phenol-pH 9-RNases method. 543–545 Grossman L., Moldave K. Methods in enzymology 12 part A Academic Press Inc.; New York:
    [Google Scholar]
  19. Otta J. D., English H. 1971; Serology and pathology of Pseudomonas syringae . Phytopathology 61:443–452
    [Google Scholar]
  20. Palleroni N. J., Ballard R. W., Ralston E., Doudoroff M. 1972; Deoxyribonucleic acid homologies among some Pseudomonas species. J. Bacteriol 110:1–11
    [Google Scholar]
  21. Palmer B., C, and Cameron H. R. 1971; Comparison of plant-pathogenic pseudomonads by disc-gel electrophoresis. Phytopathology 61:984–986
    [Google Scholar]
  22. Rogul M., Brendle J. J., Haapala D. K., Alexander A. D. 1970; Nucleic acid similarities among Pseudomonas pseudomallei Pseudomonas multi-vorans, and Actinobacillus mallei . J. Bacteriol 101:827–835
    [Google Scholar]
  23. Sands D. C, Schroth M. N., Hildebrand D. C. 1970; Taxonomy of phytopathogenic pseudomonads. J. Bacteriol 101:9–23
    [Google Scholar]
  24. Schroth M. N., Hildebrand D. C. 1972; Current taxonomic thinking on the genus Pseudomonas, with emphasis on the plant pathogens. 261–267Proceedings of the Third International Conference on Plant Pathogenic Bacteria. Centre Agr. Publ. and Document.Wageningen, The Netherlands
    [Google Scholar]
  25. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol 43:159–271
    [Google Scholar]
  26. Starr M. P. 1959; Bacteria as plant pathogens. Annu. Rev. Microbiol 13:211–238
    [Google Scholar]
  27. Stolp M., Starr M. P., Baigent N. L. 1965; Problems in speciation of phytopathogenic pseudomonads and xanthomonads. Annu. Rev. Phytopathol 3:231–264
    [Google Scholar]
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