1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 23, Issue 1

Intrageneric Relatedness of Pirie Free

Abstract

Deoxyribonucleic acid (DNA) base composition, differential biochemical properties, and DNA:DNA hybridization patterns of listeriae were studied. DNA from , and contained 38 to 40 mole percent guanine plus cytosine (% GC); however, DNA from contained 56% GC. Hybridization studies indicated that and were closely related and formed a homogeneous genetic group distinct from strains could be separated into two groups on the basis of percent reassociation values with 19303, the reference strain. One group of six strains showed 80 to 100% relative reassociation with strain 19303; four strains showed only 40 to 70% relative reassociation. There was no correlation between virulence, origin, or serotype and the degree of molecular relatedness to the reference strain. was distinct from the other three species of in biochemical properties and DNA composition, and the relative reassociation values for DNA from with DNA from other listeriae were very low. It is suggested that consideration be given to the reclassification of

  • Published Online:
© American Society for Microbiology, 1973
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-23-1-8
1973-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/23/1/ijs-23-1-8.html?itemId=/content/journal/ijsem/10.1099/00207713-23-1-8&mimeType=html&fmt=ahah

References

  1. Breed R. S., Murray E. G. D., Smith N. R. 1957; Bergey’s manual of determinative bacteriology. , 7.597–599 The Williams and Wilkins Co.; Baltimore:
     [Google Scholar]
  2. Davis G. H., Fomin L., Wilson E., Newton K. G. 1969; Numerical taxonomy of Listeria, streptococci and possibly related bacteria. J. Gen. Microbiol 57:333–348
     [Google Scholar]
  3. DeLey J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol 101:738–754
     [Google Scholar]
  4. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. Mol. Biol 12:829–842
     [Google Scholar]
  5. Jones D., Sneath P. H. A. 1970; Genetic transfer and bacterial taxonomy. Bacteriol. Rev 34:40–81
     [Google Scholar]
  6. Jones D., Sneath P. H. A., Woodbine M. 1966; The relation between Listeria and other bacteria. 161–164In Proceedings of the Third International Symposium on ListeriosisBilthoven, Holland
     [Google Scholar]
  7. Laird C. D., McConaughy B. L., McCarthy B. J. 1969; Rate of fixation of nucleotide substitutions in evolution. Nature (London) 224:149–154
     [Google Scholar]
  8. Larsen H. E., Seeliger H. P. R. 1966; A mannitol fermenting Listeria: Listeria grayi sp. n.. 35–39In Proceedings of the Third International Symposium on ListeriosisBilthoven, Holland
     [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:201–218
     [Google Scholar]
  10. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol 5:109–118
     [Google Scholar]
  11. Murray E. G. D., Webb R. A., Swann M. B. R. 1926; A disease of rabbits characterized by large mononuclear leukocytosis, caused by a hitherto undescribed bacillus: Bacterium monocytogenes (n. sp.). J. Pathol. Bacteriol 29:407–439
     [Google Scholar]
  12. Prévot A. 1961 Traité de systématique bactérienne512 Dunod; Paris:
     [Google Scholar]
  13. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol 4:430–443
     [Google Scholar]
  14. Society of American Bacteriologists 1957 Manual of microbiological methods McGraw-Hill Book Co.; New York:
     [Google Scholar]
  15. Welshimer H. J., Meredith A. L. 1971; Listeria murrayi sp. n.: a nitrate reducing mannitol fermenting Listeria . Int. J. Syst. Bacteriol 21:3–7
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-23-1-8
Loading
Intrageneric Relatedness of Listeria Pirie
Int J Syst Evol Microbiol 23, 8 (1973); https://doi.org/10.1099/00207713-23-1-8
/content/journal/ijsem/10.1099/00207713-23-1-8
/content/journal/ijsem/10.1099/00207713-23-1-8
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error