1887

Abstract

A new, extremely thermophilic bacterium from an alkaline hot spring in Yellowstone National Park is described. The organism is gram negative and occurs as pleomorphic rods, often appearing in pairs. It is an obligate aerobe and grows optimally at 70 to 75 C at a pH of 8.2 to 8.5 with a generation time of 5.5 h. When grown in culture, the organism has a very pronounced pink color due to cellular carotenoid pigments. The deoxyribonucleic acid base ratio as determined by cesium chloride density gradient ultracentrifugation was found to be 64.3 mol% guanine plus cytosine. Nutritional studies with the new organism demonstrated a more restricted growth in comparison to organisms belonging to the genus . In studies involving defined carbon sources, it was shown that the new organism grew well only on sucrose or glycerol when glutamate served as an additional nutritional source. The organism is regarded as belonging to a new genus, for which the name is proposed. This genus is placed in the family . The type species of the new genus is sp. nov., of which ATCC 27502 is the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-23-1-28
1973-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/23/1/ijs-23-1-28.html?itemId=/content/journal/ijsem/10.1099/00207713-23-1-28&mimeType=html&fmt=ahah

References

  1. Allen M.B. 1959; Studies with Cyanidium caldarium, an anomously pigmented chlorophyte. Arch. Mikrobiol 32:270–277
    [Google Scholar]
  2. Bauman A.J., Simmonds P.G. 1969; Fatty acids and polar lipids of extremely thermophilic filamentous bacterial masses from two Yellowstone Hot Springs. J. Bacteriol 98:528–531
    [Google Scholar]
  3. Bott T.L., Brock T.D. 1969; Bacterial growth rates above 90°C in Yellowstone Hot Springs. Science 164:1411–1412
    [Google Scholar]
  4. Brock T.D. 1967; Relationship between primary productivity and standing crop along a hot spring thermal gradient. Ecology 48:566–571
    [Google Scholar]
  5. Brock T.D. 1969; Microbial growth under extreme conditions. Symp. Soc. Gen. Microbiol 19:1441
    [Google Scholar]
  6. Brock T.D., Edwards M.R. 1970; Fine structure of Thermus aquaticus, an extreme thermophile. J. Bacteriol 104:509–517
    [Google Scholar]
  7. Brock T.D., Freeze H. 1969; Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J. Bacteriol 98:289–297
    [Google Scholar]
  8. Buckmire F.L.A. 1970; The physical structure of the cell wall as a differential character. Int. J. Syst. Bacteriol 20:345–360
    [Google Scholar]
  9. Castenholz R.W. 1969; Thermophilic blue-green algae and the thermal environment. Bacteriol. Rev 33:476–504
    [Google Scholar]
  10. Harding R.W., Huang P.C., Mitchell H.K. 1969; Photochemical studies of the carotenoid biosynthetic pathway in Neurospora crassa . Arch. Biochem. Biophys 129:696–707
    [Google Scholar]
  11. Hertzberg S., Liaaen-Jensen S. 1966; Bacterial Carotenoids. XIX. The carotenoids of Mycobacterium phlei strain Vera. 1. The structures of the minor carotenoids. Acta Chem. Scand 20:1187–1194
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  13. Pierson B.K., Castenholz R.W. 1971; Bac-teriochlorophylls in gliding procaryotes from hot springs. Nature N. Biol 233:25–27
    [Google Scholar]
  14. Ramaley R.F., Hixson J. 1970; Isolation of a nonpigmented, thermophilic bacterium similar to Thermus aquaticus . J. Bacteriol 103:527–528
    [Google Scholar]
  15. Schildkraut C.L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J.Mol.Biol 4:430–443
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-23-1-28
Loading
/content/journal/ijsem/10.1099/00207713-23-1-28
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error