In biological science, metagenomics has revolutionised the process of drug discovery. This is because metagenomics serves as a tool for in depth characterisation by examining, sequencing, replicating and identifying the whole DNA/genome collected from any mixed population (Mahapatra , 2019). Metagenomics is used to study Carbohydrate Active Enzymes (CAZymes) containing microbial communities due to molecular-based culture-independent methods (Kunath , 2017). CAZymes are made of multiple domains; each domain serves as the key mediator for a specific function for the protein and structure. In the Carbohydrate-Active enZYmes (CAZy) database 20% of all protein domains are identified as domains of unknown function (DUFs). DUFs are mostly ignored due to not being the most abundant in many genomes. However, in a paper by Goodacre 2014, DUFs are shown to be essential and likely related to the organism survival function due to their relation to essential proteins(Goodacre , 2014). In the CAZy database multiple DUFs are associated with catalytic CAZyme domains, which could result in them having a similar function or increase their catalytic activity. We present DUFs that are considered to have the capability to help CAZymes break down polysaccharide chains and tools/methods we used to achieve these results.

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