@article{mbs:/content/journal/acmi/10.1099/acmi.ac2019.po0562, author = "MacFadyen, Alison and Paterson, Gavin", title = "Pangenomic analysis of Staphylococcus pseudintermedius to better understand antimicrobial resistance profiles", journal= "Access Microbiology", year = "2019", volume = "1", number = "1A", pages = "", doi = "https://doi.org/10.1099/acmi.ac2019.po0562", url = "https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.ac2019.po0562", publisher = "Microbiology Society", issn = "2516-8290", type = "Journal Article", eid = "870", abstract = "Although Staphylococcus pseudintermedius are commensal bacteria of dogs, they are also opportunistic pathogens, being the primary cause of canine skin and ear infections. Recently, more attention has been given to this species, due to the emergence of antimicrobial resistance. Further to this a methicillin resistant S. pseuintermedius was isolated from a human and was subsequently found to be multidrug resistant. To better understand the frequency of carriage of antimicrobial resistance, as well as the presence of multidrug resistance, we examined the pangenome of over 200 S. pseudintermedius isolates from across the globe. Focussing on methicillin resistance we were able to identify staphylococcal cassette chromosome mec (SCCmec) types unique to specific Bayesian Analysis of Population Structure (BAPS) groups, including groups which carried mec resistance genes independent of a known SCCmec element. In addition, we identified an SCCmec element, within isolates from North America, that shares 99 % nucleotide identity with a recently described non-typeable SCCmec element carried by Staphylococcus aureus isolated from a human in the Netherlands. Beyond methicillin resistance we found over 50 % of those strains analysed were putatively multidrug resistant (resistant to 3 or more antimicrobial drug classes). This highlights the diverse resistance determinants present in animal staphylococci and the importance of monitoring S. pseudintermedius.", }