1887

Abstract

Campylobacter jejuniis is the most common cause of bacterial gastroenteritis worldwide. It is often considered a ‘commensal’ in chickens where it rapidly colonises the caecum of young chicks and is present in around 80 % of farmed poultry. C. jejuni has a small genome and most strains are unable to transport or metabolize glucose. Instead they use amino acids, TCA cycle intermediates and short chain fatty acids as energy and carbon sources. Recently, a WGS project identified an unusual group of C. jejuni (RG-2 group) within a bank of C. jejuni strains isolated from farm associated Norway rats. These strains had acquired an entire locus (glc) of seven genes which enable uptake and metabolism of glucose via the Entner Doudoroff (ED) pathway. This project is addressing the impact of these genes on metabolism and niche survival of these strains. Campylobacter are microaerophilic bacteria. Good growth of C. jejuni NCTC 11168 and of aglcnegative Norway rat C. jejuni isolate was recorded in the presence of 5 % O2up to 13 %in a Whitley M35 workstation. In contrast, two glcpositive strains of C. jejuni, Dg275 and Dg95, exhibited the unusual ability to grow well in an atmosphere of 16 % oxygen, as monitored by c.f.u. and OD600. Current studies are also focusing on the contribution of glucose utilization via the ED pathway to influence survival and growth in oxygen.

Loading

Article metrics loading...

/content/journal/acmi/10.1099/acmi.ac2019.po0241
2019-04-08
2020-01-17
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/acmi/10.1099/acmi.ac2019.po0241
Loading

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error