RT Journal Article SR Electronic(1) A1 Kiu, Raymond A1 Caim, Shabhonam A1 Pickard, Derek A1 Dougan, Gordon A1 Painset, Anais A1 Swift, Craig A1 Mather, Alison A1 Amar, Corinne A1 Hall, LindsayYR 2019 T1 Phylogenomic analysis of gastroenteritis-associated Clostridium perfringens identifies isogenic strains in multiple outbreaks and novel virulence-related features JF Access Microbiology, VO 1 IS 1A OP SP 211 DO https://doi.org/10.1099/acmi.ac2019.po0080 PB Microbiology Society, SN 2516-8290, AB Background Clostridium perfringensis a major enteric pathogen known to cause gastroenteritis in humans. Although major outbreak cases are frequently reported, to date no Whole Genome Sequencing (WGS) based studies have been performed to understand the genomic epidemiology and virulence gene content of C. perfringens-associated outbreak strains. Methods We have applied both genomic and phylogenetic analyses on a sub-set of 109 newly-sequenced C. perfringens strains isolated from gastroenteritis-associated disease cases (including food-poisoning and care-home diarrhoea) from England and Wales between 2011 – 2017 to probe virulence profiles including toxin and AMR genes, plasmid features and genomic epidemiology of these case isolates. Results Our data identified that highly-similar C. perfringens strains were associated with 9 care home-associated individual outbreaks over a 5 year interval, indicating potential common sources linked to these distinct outbreaks. Enterotoxin gene cpe was encoded in all but 4 isolates (96.4 % type-F strains), and it was further determined that virulence plasmids encoding cpe were extensively distributed in the isolates (97 % care-home isolates carry pCPF5603 plasmid; 60 % food-poisoning isolates carry pCPF4969 plasmid). Further virulence factors, such as β2-toxin, were enriched in these isolates (46.7 %). Phage proteins were also commonly identified, with additional analysis indicating phages may contribute to spread of virulence determinants. Conclusion This study highlights the genotypic and epidemiological relatedness of a large collection of C. perfringens strains isolated from gastroenteritis-associated cases from across the UK and Wales. Key points revealed include the potential circulation of disease-associated strains, and impact of cpe-encoding-plasmid disseminations, linked to outbreak cases. , UL https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.ac2019.po0080