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Volume 8, Issue 2

Genome sequences of distinct genotypes of bacterial pathogen pv. from pepper ( L.) in Serbia Open Access

Abstract

This Technical Resource presents genome sequence data for three strains of the bacterial pathogen pv. () collected in Serbia. We isolated these strains from pepper crops showing bacterial spot symptoms in 2016 at the municipality of Irig, in the Srem district. The presented data comprise raw sequencing reads and annotated, contig-level genome assemblies. We checked for the presence of sequences of known type-3 secretion system (T3SS) effector genes and plasmid-like sequences. Phylogenomic reconstruction revealed that the three strains fell in the same clade within . Strain X13 is most closely related to strain 66b, collected in Bulgaria in 2012. Strains X22 and X31 are most closely related to Tu-10 collected in the Southeastern Anatolia region of Türkiye in 2020. In common with other members of the clade, all three strains share a 75 kb plasmid that carries T3SS effector genes , , and . Additionally, strain X13 shares extensive sequence similarity to the pXCV183 plasmid, including T3SS effector gene , and shares extensive sequence similarity with plasmid pXap41, including T3SS effector gene . This difference in plasmid content might contribute to the observed difference in virulence among the Serbian strains. The three Serbian strains lack a 31 kb plasmid, pLMG730.4, that is seen in several Vietnamese and Canadian strains within this clade of . The data presented will be a useful resource for future molecular epidemiology and genomic surveillance of this pathogen in the Balkan region, augmenting the previously available draft genome sequences of strains 66b (Bulgaria) and 83M (North Macedonia).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Capsicum annuum L. , genome sequence , Illumina sequencing , pathogenicity , pepper , plasmid , Serbia , Unicycler assembly , Xanthomonas campestris pv. vesicatoria group A and Xanthomonas euvesicatoria pv. euvesicatoria
Funding
This study was supported by the:
  • UK Research and Innovation (Award BB/T010916/1)
    • Principal Award Recipient: DavidStudholme
  • Biotechnology and Biological Sciences Research Council (Award BB/W018853/1)
    • Principal Award Recipient: MurrayGrant
  • Wellcome Trust (Award 218247/Z/19/Z)
    • Principal Award Recipient: NotApplicable
  • Innovation Fund of the Republic of Serbia (Award 451-03-136/2025-03/200010)
    • Principal Award Recipient: TatjanaPopovic Milovanovic
  • Innovation Fund of the Republic of Serbia (Award 451-03-136/2025-03/200053)
    • Principal Award Recipient: TatjanaPopovic Milovanovic
  • Innovation Fund of the Republic of Serbia (Award 451-03-136/2025-03/200117)
    • Principal Award Recipient: TatjanaPopovic Milovanovic
© 2026 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2026-02-09

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/content/journal/acmi/10.1099/acmi.0.001138.v4
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Genome sequences of distinct genotypes of bacterial pathogen Xanthomonas euvesicatoria pv. euvesicatoria from pepper (Capsicum annuum L.) in Serbia
Access Microbiology 8, 001138.v4 (2026); https://doi.org/10.1099/acmi.0.001138.v4
/content/journal/acmi/10.1099/acmi.0.001138.v4
/content/journal/acmi/10.1099/acmi.0.001138.v4
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