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Volume 8, Issue 1

Detection of , and gene encoding aminoglycoside resistance in in Burkina Faso Open Access

Abstract

is the second most frequently isolated bacterium in medical bacteriology laboratories after . It can be responsible for many infections, including urinary tract infections and pneumonia. The treatment of these infections is often prolonged because of the resistance of this bacterium to antibiotics. The production of aminoglycoside-modifying enzymes and the production of RNA methylases confer resistance to aminoglycosides. In Burkina Faso, studies on bacterial resistance to aminoglycosides, an antibiotic regularly prescribed and consumed by patients, are limited; hence, the purpose of this study was to determine the prevalence of aminoglycoside resistance genes in isolated from urine and pus samples.

A total of 150 strains from pus and urine cultures were collected from October 2018 to June 2019 in two hospitals in Ouagadougou and were included in this study. After plating on Muller–Hinton medium to obtain pure colonies, antibiotic susceptibility testing was performed with aminoglycosides, -lactams, fluoroquinolones and sulphonamides. PCR testing for the gene allowed genotypic identification of . PCRs were then performed on strains with at least one aminoglycoside resistance for identification of the , and resistance genes.

Antibiotic susceptibility testing showed that 38.67% (58/150) of the strains were resistant to tobramycin, 28.67% (43/150) to gentamicin, 10.00% (15/150) to netilmicin, 8.67% (13/150) to kanamycin, 4.67% (7/150) to amikacin and 0.67% (1/150) to neomycin. Of the 63 strains (42.66%) with at least 1 aminoglycoside resistance, the resistance genes, and , were detected in 49 strains (77.77%) and 39 strains (61.90%), respectively, and 34 strains (53.97%) had both genes. The gene was present in 19.04% of the strains (12/63).

Aminoglycoside resistance in strains in this study is primarily due to and acetyltransferases. Although RNA methylase genes are less frequent, increased surveillance is necessary due to the high level of aminoglycoside resistance conferred by this gene.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): aac(3)-IIc , aac(6′)Ib , aminoglycoside resistance , armA , Burkina Faso and Klebsiella pneumoniae
© 2026 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2026-01-07
2026-01-22

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Detection of aac(3)IIc, aac(6)Ib and armA gene encoding aminoglycoside resistance in Klebsiella pneumoniae in Burkina Faso
Access Microbiology 8, 001115.v3 (2026); https://doi.org/10.1099/acmi.0.001115.v3
/content/journal/acmi/10.1099/acmi.0.001115.v3
/content/journal/acmi/10.1099/acmi.0.001115.v3
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