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Volume 7, Issue 12

Gut microbiota composition in preterm infants with and without necrotizing enterocolitis: a systematic review and narrative synthesis Open Access

Abstract

Necrotizing enterocolitis (NEC) is a devastating gastrointestinal disorder in preterm infants with a high mortality rate. The aetiology of NEC appears to be multifaceted; however, gut microbiota dysbiosis likely plays a significant role. This systematic review aimed to describe how the gut microbiota of preterm infants with NEC differs from infants without NEC (PROSPERO: CRD42022344126). Databases were searched from inception to 22 June 2022 to identify eligible studies that examined the gut microbiota composition of preterm infants with and without NEC using sequencing methods. Results were described narratively. We identified 28 eligible studies. Overall, findings were heterogeneous and no single gut microbiota signature was associated with NEC in all studies. Importantly, 3 studies reported no difference in the gut microbiota composition between NEC and healthy infants, while studies reported a difference using one or more analytical method (i.e. alpha diversity, beta diversity or differential abundance analysis). Of note, NEC (or development of NEC) was positively associated with increased detection and/or abundance of (=11 studies), (=8) and Proteobacteria (=2). The taxa most frequently associated with NEC (, , and Proteobacteria) may play an important role in the pathogenesis of NEC and should be further explored.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Clostridium , Enterobacteriaceae , gut microbiota , necrotizing enterocolitis , preterm and Proteobacteria
Funding
This study was supported by the:
  • National Health and Medical Research Council (Award 1197951)
    • Principal Award Recipient: SuzanneGarland
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2025-12-19
2026-02-18

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Gut microbiota composition in preterm infants with and without necrotizing enterocolitis: a systematic review and narrative synthesis
Access Microbiology 7, 001077.v3 (2025); https://doi.org/10.1099/acmi.0.001077.v3
/content/journal/acmi/10.1099/acmi.0.001077.v3
/content/journal/acmi/10.1099/acmi.0.001077.v3
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