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Abstract

Lightly pickled vegetables have gained popularity as a healthier alternative to traditional high-salt pickles; however, their low salt content increases the risk of foodborne pathogen proliferation. In this study, we aimed to investigate the antibacterial effects of Chinese chive extract (CCE) against using culture media and lightly pickled napa cabbage as model systems. The results revealed that CCE exhibited concentration-dependent inhibitory effects on growth. On agar media, ≥3% CCE completely suppressed colony formation. In broth media, ≥3% CCE clearly inhibited proliferation at both 10 and 35 °C. Furthermore, in lightly pickled cabbage treated with CCE, the growth of coliform and total aerobic bacteria was significantly suppressed at both refrigeration and ambient temperatures. Thus, CCE could serve as a promising natural antimicrobial agent in the production of lightly pickled vegetables, offering a strategy to potentially enhance food safety, independent of synthetic food additives.

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/content/journal/acmi/10.1099/acmi.0.001073.v3
2026-04-10
2026-04-14

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References

  1. Kataoka I, Eko M, Terashima H, Higashi K, Nakasuji Y et al. A diffuse outbreak of enterohemorrhagic E. coli O157 by lightly-pickled vegetables. Jpn J Food Microbiol 2013; 30:112–115 [View Article]
    [Google Scholar]
  2. Tabuchi A, Wakui T, Yahata Y, Yano K, Azuma K et al. A large outbreak of enterohaemorrhagic Escherichia coli O157, caused by low-salt pickled Napa cabbage in nursing homes, Japan, 2012. Western Pac Surveill Response J 2015; 6:7–11 [View Article] [PubMed]
    [Google Scholar]
  3. Uehara R, Kuramochi K, Akasaka M, Fukuda K, Amagai A et al. An outbreak of verotoxin-producing Escherichia coli O157:H7 infection caused by lightly salted turnips in a nursing home for aged, June 2000 – Saitama. IASR 2000; 21:272–273
    [Google Scholar]
  4. Oda T, Furuta M, Hiwaki H, Ozaki N, Ohba M et al. Fate of Escherichia coli O157 in the production process of Asazuke (salted cucumber). Jpn J Food Microbiol 2004; 21:275–280 [View Article]
    [Google Scholar]
  5. Saito A. Enterohemorrhagic Escherichia coli food poisoning—recent 10 years of occurrence, issues and countermeasures—. Jpn J Food Microbiol 2020; 37:112–118 [View Article]
    [Google Scholar]
  6. Chinchkar AV, Singh A, Singh SV, Acharya AM, Kamble MG. Potential sanitizers and disinfectants for fresh fruits and vegetables: a comprehensive review. J Food Process Preserv 2022; 46:e16495 [View Article]
    [Google Scholar]
  7. Deng L-Z, Mujumdar AS, Pan Z, Vidyarthi SK, Xu J et al. Emerging chemical and physical disinfection technologies of fruits and vegetables: a comprehensive review. Crit Rev Food Sci Nutr 2020; 60:2481–2508 [View Article] [PubMed]
    [Google Scholar]
  8. Álvarez-Martínez FJ, Barrajón-Catalán E, Herranz-López M, Micol V. Antibacterial plant compounds, extracts and essential oils: an updated review on their effects and putative mechanisms of action. Phytomedicine 2021; 90:153626 [View Article] [PubMed]
    [Google Scholar]
  9. Kudo H, Takeuchi H, Shimamura T, Kadota Y, Sugiura T et al. In vitro anti-Helicobacter pylori activity of Chinese chive (Allium tuberosum). FSTR 2011; 17:505–513 [View Article]
    [Google Scholar]
  10. Mau J-L, Chen C-P, Hsieh P-C. Antimicrobial effect of extracts from Chinese chive, cinnamon, and Corni Fructus. J Agric Food Chem 2001; 49:183–188 [View Article]
    [Google Scholar]
  11. Mnayer D, Fabiano-Tixier A-S, Petitcolas E, Hamieh T, Nehme N et al. Chemical composition, antibacterial and antioxidant activities of six essentials oils from the Alliaceae family. Molecules 2014; 19:20034–20053 [View Article] [PubMed]
    [Google Scholar]
  12. Seo KI, Moon YH, Choi SU, Park KH. Antibacterial activity of S-methyl methanethiosulfinate and S-methyl 2-propene-1-thiosulfinate from Chinese chive toward Escherichia coli O157:H7. Biosci Biotechnol Biochem 2001; 65:966–968 [View Article] [PubMed]
    [Google Scholar]
  13. Gao Q, Li X-B, Sun J, Xia E-D, Tang F et al. Isolation and identification of new chemical constituents from Chinese chive (Allium tuberosum) and toxicological evaluation of raw and cooked Chinese chive. Food Chem Toxicol 2018; 112:400–411 [View Article] [PubMed]
    [Google Scholar]
  14. Tang X, Olatunji OJ, Zhou Y, Hou X. Allium tuberosum: antidiabetic and hepatoprotective activities. Food Res Int 2017; 102:681–689 [View Article] [PubMed]
    [Google Scholar]
  15. Student The probable error of a mean. Biometrika 1908; 6:1–25 [View Article]
    [Google Scholar]
  16. Critzer FJ, Doyle MP. Microbial ecology of foodborne pathogens associated with produce. Curr Opin Biotechnol 2010; 21:125–130 [View Article] [PubMed]
    [Google Scholar]
  17. Berger CN, Sodha SV, Shaw RK, Griffin PM, Pink D et al. Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environ Microbiol 2010; 12:2385–2397 [View Article] [PubMed]
    [Google Scholar]
  18. Hamilton AJ, Stagnitti F, Premier R, Boland AM, Hale G. Quantitative microbial risk assessment models for consumption of raw vegetables irrigated with reclaimed water. Appl Environ Microbiol 2006; 72:3284–3290 [View Article] [PubMed]
    [Google Scholar]
  19. Tyrrel SF, Knox JW, Weatherhead EK. Microbiological water quality requirements for salad irrigation in the United Kingdom. J Food Prot 2006; 69:2029–2035 [View Article] [PubMed]
    [Google Scholar]
  20. Ingham SC, Losinski JA, Andrews MP, Breuer JE, Breuer JR et al. Escherichia coli contamination of vegetables grown in soils fertilized with noncomposted bovine manure: garden-scale studies. Appl Environ Microbiol 2004; 70:6420–6427 [View Article] [PubMed]
    [Google Scholar]
  21. Ongeng D, Geeraerd AH, Springael D, Ryckeboer J, Muyanja C et al. Fate of Escherichia coli O157:H7 and Salmonella enterica in the manure-amended soil-plant ecosystem of fresh vegetable crops: a review. Crit Rev Microbiol 2015; 41:273–294 [View Article] [PubMed]
    [Google Scholar]
  22. Talley JL, Wayadande AC, Wasala LP, Gerry AC, Fletcher J et al. Association of Escherichia coli O157:H7 with filth flies (Muscidae and Calliphoridae) captured in leafy greens fields and experimental transmission of E. coli O157:H7 to spinach leaves by house flies (Diptera: Muscidae). J Food Prot 2009; 72:1547–1552 [View Article] [PubMed]
    [Google Scholar]
  23. Ensink JHJ, Mahmood T, Dalsgaard A. Wastewater‐irrigated vegetables: market handling versus irrigation water quality. Tropical Med Int Health 2007; 12:2–7 [View Article]
    [Google Scholar]
  24. Kobayashi M, Suzuki Y, Katoh R, Murauchi K, Higuchi Y et al. Bactericidal effect of hot water on cucumbers contaminated with Escherichia coli. Jpn J Food Microbiol 2018; 35:41–44 [View Article]
    [Google Scholar]
  25. Maidment C, Dembny Z, Watts DI. The anti-bacterial activity of 12 alliums against Escherichia coli. J Nutr Food Sci 2001; 31:238–241 [View Article]
    [Google Scholar]
  26. Chen C, Cai J, Ren Y, Xu Y, Liu H et al. Antimicrobial activity, chemical composition and mechanism of action of Chinese chive (Allium tuberosum Rottler) extracts. Front Microbiol 2022; 13:1028627 [View Article]
    [Google Scholar]
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