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Volume 8, Issue 3

Molecular characterization of extended-spectrum beta-lactamase-producing bacteria isolated from pregnant women’s urine at Itojo Hospital, South Western Uganda Open Access

Abstract

Extended-spectrum -lactamase (ESBL)-producing bacteria pose a global challenge because of resistance developing against a wide range of antimicrobial agents, complicating available treatment options. Thus, identifying the prevalent bacterial species producing ESBL enzymes and understanding how they are susceptible to antibiotics is necessary to inform effective treatment guidelines.

We sought to characterize ESBL-producing bacteria isolated from pregnant women’s urine at Itojo Hospital, Ntungamo district, Southwestern Uganda.

We conducted a cross-sectional study where we collected and analysed 340 urine samples from 340 pregnant women. We did antimicrobial susceptibility testing using the Kirby–Bauer disc diffusion method. Isolates were screened for ESBL production and confirmed using the combination disc test. Genotypic characterization was confirmed using multiplex PCR to detect TEM, CTX-M and SHV genes.

The prevalence of ESBL-producing bacteria was 29.7% (101/340). 36/101 (35.6%) and species 33/101 (32.7%) were predominant ESBL producers. Genotypic analysis revealed TEM 50/101 (49.5%) and CTX-M 31/101 (30.7%) as the most prevalent genes, while SHV was less common, 8/101 (7.9%)

The high prevalence of ESBL-producing bacteria and their resistance to commonly used antibiotics highlighted the need for targeted antibiotic therapy, antimicrobial stewardship and regular molecular surveillance.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antenatal clinic , antimicrobial , extended spectrum β-lactamase and pregnancy
© 2026 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2026-03-11
2026-04-13

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References

  1. Wagenlehner FME, Bjerklund Johansen TE, Cai T, Koves B, Kranz J et al. Epidemiology, definition and treatment of complicated urinary tract infections. Nat Rev Urol 2020; 17:586–600 [View Article]
     [Google Scholar]
  2. Belete MA. Bacterial profile and ESBL screening of urinary tract infection among asymptomatic and symptomatic pregnant women attending antenatal care of Northeastern Ethiopia Region. Infect Drug Resist 2020; 13:2579–2592 [View Article] [PubMed]
     [Google Scholar]
  3. Ngong IN, Fru-Cho J, Yung MA, Akoachere J-FKT. Prevalence, antimicrobial susceptibility pattern and associated risk factors for urinary tract infections in pregnant women attending ANC in some integrated health centers in the Buea health district. BMC Pregnancy Childbirth 2021; 21:673 [View Article] [PubMed]
     [Google Scholar]
  4. Venkatesh A, Sanchez-Samaniego G, Mäusezahl D, Hattendorf J, Hartinger SM. Prevalence of urinary tract infections in pregnancy in rural Andean communities of Peru. Womens Health (Lond Engl) 2024; 20:17455057241294215 [View Article]
     [Google Scholar]
  5. Johnson B, Stephen BM, Joseph N, Asiphas O, Musa K et al. Prevalence and bacteriology of culture-positive urinary tract infection among pregnant women with suspected urinary tract infection at Mbarara regional referral hospital, South-Western Uganda. BMC Pregnancy Childbirth 2021; 21:159 [View Article] [PubMed]
     [Google Scholar]
  6. Amiri M, Lavasani Z, Norouzirad R, Najibpour R, Mohamadpour M et al. Prevalence of urinary tract infection among pregnant women and its complications in their newborns during the birth in the hospitals of Dezful city, Iran, 2012 - 2013. Iran Red Crescent Med J 2015; 17:e26946 [View Article] [PubMed]
     [Google Scholar]
  7. Tacconelli E, Carrara E, Savoldi A, Harbarth S, Mendelson M et al. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect Dis 2018; 18:318–327 [View Article] [PubMed]
     [Google Scholar]
  8. Bader MS, Loeb M, Brooks AA. An update on the management of urinary tract infections in the era of antimicrobial resistance. Postgrad Med 2017; 129:242–258 [View Article] [PubMed]
     [Google Scholar]
  9. Sher EK, Džidić-Krivić A, Sesar A, Farhat EK, Čeliković A et al. Current state and novel outlook on prevention and treatment of rising antibiotic resistance in urinary tract infections. Pharmacol Ther 2024; 261:108688 [View Article] [PubMed]
     [Google Scholar]
  10. Waller TA, Pantin SAL, Yenior AL, Pujalte GGA. Urinary tract infection antibiotic resistance in the United States. Prim Care Clin Off Pract 2018; 45:455–466 [View Article]
     [Google Scholar]
  11. Frost I, Van Boeckel TP, Pires J, Craig J, Laxminarayan R. Global geographic trends in antimicrobial resistance: the role of international travel. J Travel Med 2019; 26:taz036 [View Article] [PubMed]
     [Google Scholar]
  12. Teklu DS, Negeri AA, Legese MH, Bedada TL, Woldemariam HK et al. Extended-spectrum beta-lactamase production and multi-drug resistance among Enterobacteriaceae isolated in Addis Ababa, Ethiopia. Antimicrob Resist Infect Control 2019; 8:39 [View Article] [PubMed]
     [Google Scholar]
  13. Kazemian P, Shebl FM, McCann N, Walensky RP, Wexler DJ. Evaluation of the cascade of diabetes care in the United States, 2005-2016. JAMA Intern Med 2019; 179:1376–1385 [View Article] [PubMed]
     [Google Scholar]
  14. Youssef MM, Rizk HA, Hassuna NA. Phenotypic and genotypic characterization of extended-spectrum β-Lactamase-producing enterobacteriaceae in asymptomatic bacteriuria in pregnancy. Microb Drug Resist 2019; 25:731–738 [View Article] [PubMed]
     [Google Scholar]
  15. Moses A, Bwanga F, Boum Y, Bazira J. Prevalence and genotypic characterization of extended-spectrum Beta-Lactamases produced by gram negative bacilli at a tertiary care hospital in rural South Western Uganda. Br Microbiol Res J 2014; 4:1541–1550 [View Article] [PubMed]
     [Google Scholar]
  16. Verma S, Kalyan RK, Gupta P, Khan MD, Venkatesh V. Molecular characterization of extended spectrum β-Lactamase producing Escherichia coli and Klebsiella pneumoniae isolates and their antibiotic resistance profile in health care-associated urinary tract infections in North India. J Lab Physicians 2023; 15:194–201 [View Article] [PubMed]
     [Google Scholar]
  17. Jena J, Sahoo RK, Debata NK, Subudhi E. Prevalence of TEM, SHV, and CTX-M genes of extended-spectrum β-lactamase-producing Escherichia coli strains isolated from urinary tract infections in adults. 3 Biotech 2017; 7:244 [View Article] [PubMed]
     [Google Scholar]
  18. Husna A, Rahman MM, Badruzzaman ATM, Sikder MH, Islam MR et al. Extended-spectrum β-lactamases (ESBL): challenges and opportunities. Biomedicines 2023; 11:2937 [View Article] [PubMed]
     [Google Scholar]
  19. Elsayed AGA, Badr DF, El Kheir NYA, Zaki MES, Mossad AEM et al. Prevalence of extended-spectrum beta-lactamase and molecular detection of blaTEM, blaSHV, and blaCTX-M genotypes among gram-negative Bacilli isolates from hospital acquired infections in pediatrics, one institutional study. Ital J Pediatr 2024; 50:31 [View Article] [PubMed]
     [Google Scholar]
  20. Chaudhary MK, Jadhav I, Banjara MR. Molecular detection of plasmid mediated blaTEM, blaCTX−M,and blaSHV genes in extended spectrum β-Lactamase (ESBL) Escherichia coli from clinical samples. Ann Clin Microbiol Antimicrob 2023; 22:33 [View Article]
     [Google Scholar]
  21. Murray CJL, Ikuta KS, Sharara F, Swetschinski L, Robles Aguilar G et al. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. The Lancet 2022; 399:629–655 [View Article]
     [Google Scholar]
  22. Singhal T. “Rationalization of Empiric Antibiotic Therapy” - A move towards preventing emergence of resistant infections. Indian J Pediatr 2020; 87:945–950 [View Article] [PubMed]
     [Google Scholar]
/content/journal/acmi/10.1099/acmi.0.001045.v5
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Molecular characterization of extended-spectrum beta-lactamase-producing bacteria isolated from pregnant women’s urine at Itojo Hospital, South Western Uganda
Access Microbiology 8, 001045.v5 (2026); https://doi.org/10.1099/acmi.0.001045.v5
/content/journal/acmi/10.1099/acmi.0.001045.v5
/content/journal/acmi/10.1099/acmi.0.001045.v5
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