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Volume 8, Issue 4

Draft genome sequences of four spp. isolates, including one potential new species, from tropical legume plants in Costa Rica Open Access

Abstract

We present the draft genomes of four strains of isolated from root nodules of , , and in Costa Rica. Through comparative genomics, including the estimation of the average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH) and phylogenomic analysis, we determined that sp LEGMi-135b represents a potentially novel species near (ANI: 94%; dDDH: 53.2%). The strain LEGMi-12c was associated with (ANI: 98%; dDDH: 87%), strain LEGMi-166a to (ANI: 100%; dDDH: 99.3%) and strain LEGMi-198b was related to (ANI: 98%; dDDH: 81.4%). Using functional annotation tools, we also determined the presence of genes related to nodulation and nitrogen fixation, such as , , , and , in each strain. With this work, we provide valuable genomic resources of the genus that will be useful for future studies on rhizobial taxonomy, symbiosis and ecology in tropical ecosystems.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Fabaceae , nitrogen fixation , nodulation , Rhizobiaceae and symbiosis
Funding
This study was supported by the:
  • Vicerrectoría de Investigación, Universidad de Costa Rica (Award B9-204 and C0-524)
    • Principal Award Recipient: KeilorRojas-Jimenez
  • Ministerio de ciencia, innovación, tecnología y telecomunicaciones de Costa Rica (MICITT)) (Award FI-036B-19)
    • Principal Award Recipient: KeilorRojas-Jimenez
© 2026 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2026-04-02
2026-04-11

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/content/journal/acmi/10.1099/acmi.0.001012.v3
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Draft genome sequences of four Rhizobium spp. isolates, including one potential new species, from tropical legume plants in Costa Rica
Access Microbiology 8, 001012.v3 (2026); https://doi.org/10.1099/acmi.0.001012.v3
/content/journal/acmi/10.1099/acmi.0.001012.v3
/content/journal/acmi/10.1099/acmi.0.001012.v3
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