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Abstract

is responsible for 80% of the cases of human foodborne bacterial enteric infections worldwide. However, limited data on its genetic diversity exist, especially using the enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The study aimed to determine the genetic diversity of strains isolated from infant diarrhoeal faeces at the Omar Bongo Ondimba Regional Health Center of Makokou (CHROBOM), Gabon.

A total of 58 strains of from patients with gastroenteritis were used in this study. The ERIC-PCR method was used to characterize genetic diversity. The binomial manual method via the online analysis system (http://insilico.ehu.es/dice_upgma/) was used to establish the dendrogram and calculate the discriminatory power of the Simpson diversity index ().

The genotyping of isolates by the ERIC-PCR method revealed a discriminatory index =0.8451, dividing the 58 isolates into 10 clusters, with 33 genotypic profiles, including 22 non-repeated profiles and 11 repeated profiles. These results indicate a rather polymorphic diversity of in the Makokou region of Gabon.

The high discriminatory diversity index obtained in this study demonstrates the polymorphic richness within strains as revealed by the ERIC-PCR method.

  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. The Microbiology Society waived the open access fees for this article.
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/content/journal/acmi/10.1099/acmi.0.000947.v3
2025-03-14
2025-06-23
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