1887

Abstract

Conjugation of plasmids from is essential for the genetic manipulation of spp. To facilitate intergeneric conjugation from to the conjugative machinery required for genetic transfer is usually provided by the non-transferable helper plasmid, pUZ8002. Here we present the complete nucleotide sequence of pUZ8002, describe the previously undocumented creation process, and provide details of the sequence relative to the parental pUZ8 plasmid and another previously published pUZ8002 sequence.

Funding
This study was supported by the:
  • Royal Academy of Engineering Research Chair Scheme (Award RCSRF2021\11\15)
    • Principle Award Recipient: PaulA Hoskisson
  • Biotechnology and Biological Sciences Research Council (Award BB/T004126/1)
    • Principle Award Recipient: PaulA Hoskisson
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-06-05
2024-06-19
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