Conjugation of plasmids from Escherichia coli is essential for the genetic manipulation of Streptomyces spp. To facilitate intergeneric conjugation from E. coli to Streptomyces the conjugative machinery required for genetic transfer is usually provided by the non-transferable helper plasmid, pUZ8002. Here we present the complete nucleotide sequence of pUZ8002, describe the previously undocumented creation process, and provide details of the sequence relative to the parental pUZ8 plasmid and another previously published pUZ8002 sequence.

This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/T004126/1)
    • Principle Award Recipient: Paul A Hoskisson
  • Royal Academy of Engineering Research Chair Scheme (Award RCSRF2021\11\15)
    • Principle Award Recipient: Paul A Hoskisson
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.

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