Version 2: Biofilm destruction activity of alpha-tocopherol against Staphylococcus aureus, Proteus mirabilis and Pseudomonas aeruginosa

Antibiotic resistance and the persistence of sessile cells within biofilms complicate the eradication of biofilm-related infections using conventional antibiotics. This highlights the necessity for alternate therapy methods. The objective of this study was to investigate the biofilm destruction activity of alpha-tocopherol against Staphylococcus aureus, Proteus mirabilis and Pseudomonas aeruginosa on polystyrene. Alpha-tocopherol showed significant biofilm destruction activity on the pre-formed biofilms of S. aureus (45 - 46%), P. mirabilis (42 - 54%) and P. aeruginosa (28%). Resazurin assay showed that alpha-tocopherol disrupted all bacteria biofilms without interfering with their cell viability. Scanning electron microscope images showed lower bacterial cell count and less compacted cell aggregates on polystyrene surfaces after treatment with alpha-tocopherol. This study demonstrated the biofilm destruction activity of alpha-tocopherol against S. aureus, P. mirabilis and P. aeruginosa. Alpha-tocopherol could potentially be used to decrease biofilm-associated infections of these bacteria.