1887

Abstract

is a red alga that is commonly utilized in the Philippines as food and as a source of high-value natural products for industrial applications. However, there are no studies regarding the microbial community associated with and its potential applications. This study aimed to isolate and identify the epiphytic bacteria of and determine their antimicrobial and quorum sensing inhibitory (QSI) effects. The thalli of were collected at the shores of Santa Fe, Bantayan, Cebu, Philippines. Bacterial isolates were identified using 16S rRNA, and their ethyl acetate (EtOAc) extracts were subjected to antimicrobial susceptibility tests against representative species of yeast and Gram-negative and Gram-positive bacteria. Their QSI activity against was also determined. Fourteen distinct bacterial colonies belonging to four genera, namely (3), (5), (1) and (5), were successfully isolated and identified. All 14 bacterial isolates exhibited antibacterial effects. EPB9, identified as , consistently showed the strongest inhibition against , and , with minimum inhibitory concentrations (MICs) ranging from 0.0625 to 1.0 mg ml. In contrast, all 14 isolates showed weak antifungal effects. Both (EPB9) and (EPB15) exhibited QSI effects at 100 mg ml, showing opaque zones of 3.1±0.9 and 3.8±0.4 mm, respectively. This study is the first to isolate and identify the distinct microbial epiphytic bacterial community of and its potential as an abundant resource for new antibacterial and QSI bioactives.

Funding
This study was supported by the:
  • Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development
    • Principle Award Recipient: PaulJohn Geraldino
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-12-06
2024-02-25
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