1887

Abstract

Skin flora bacteria, such as , are the predominant contaminants of blood products used for transfusion. Platelet concentrates (PCs), a therapeutic product used to treat patients with platelet deficiencies, are stored at ambient temperature under agitation, providing ideal conditions for bacterial proliferation. At Canadian Blood Services, PCs are screened for microbial contamination using the automated BACT/ALERT culture system. Positive cultures are processed and contaminating organisms are identified using the VITEK 2 system. Over a period of approximately 2 years, several PC isolates were identified as to a high level of confidence. However, since is associated with bacterial vaginosis and is not a common PC contaminant, a retrospective investigation revealed that in all cases was misidentified as . Our investigation demonstrated that the media type used to grow PC bacterial isolates can have a significant impact on the results obtained on the VITEK 2 system. Furthermore, other identification methods such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALD-TOF MS) and PCR amplification of the 16S RNA gene were only partially successful in the identification of . Therefore, our findings support a multiphasic approach when PC isolates are identified as by the VITEK 2 system for proper identification of using macroscopic, microscopic and other biochemical analyses.

Funding
This study was supported by the:
  • Canadian Blood Services (Award Not applicable)
    • Principle Award Recipient: SandraRamirez-Arcos
  • Health Canada (Award Not applicable)
    • Principle Award Recipient: SandraRamirez-Arcos
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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/content/journal/acmi/10.1099/acmi.0.000539.v3
2023-06-16
2024-07-17
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