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Volume 5, Issue 6

Metabolic perturbations and key pathways associated with the bacteriostatic activity of flower anthocyanin fraction against Open Access

Abstract

flowers are rich in anthocyanins and possess various biological activities. Specifically, the antibacterial mechanism of action of anthocyanins remains unknown and was investigated in . A time–kill assay was used to assess the antibacterial activity and the metabolic perturbations in were investigated utilizing liquid chromatography–mass spectrometry (LC-MS)-based metabolomics. Pathway analyses were carried out for metabolites showing ≥2-fold changes. The anthocyanin fraction remarkably reduced the growth of at 4 h by 95.8 and 99.9 % at minimum inhibitory concentration (MIC) and 2× MIC, respectively. The anthocyanin fraction (MIC) had a bacteriostatic effect and was shown to have perturbed glycerophospholipids (1-acyl-sn-glycero-3-phosphoethanolamine, phosphatidylglycerol, diacylglycerol and cardiolipin), amino acids (valine, tyrosine and isoleucine) and energy (ubiquinone and NAD) metabolites at 1 and 4 h. This study demonstrated significant metabolic perturbations of the glycerophospholipid, amino acid and energy metabolism, with these being the key pathways involved in the bacteriostatic activity of anthocyanins from , which may have promise as bacteriostatic agents for -related infections.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): acylated anthocyanin , antimicrobial , blue pea flower , infectious diseases , metabolomics and natural product
Funding
This study was supported by the:
  • School of Science, Monash University Malaysia
    • Principal Award Recipient: WeeSim Choo
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-06-28
2026-04-16

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/content/journal/acmi/10.1099/acmi.0.000535.v5
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Metabolic perturbations and key pathways associated with the bacteriostatic activity of Clitoria ternatea flower anthocyanin fraction against Escherichia coli
Access Microbiology 5, 000535.v5 (2023); https://doi.org/10.1099/acmi.0.000535.v5
/content/journal/acmi/10.1099/acmi.0.000535.v5
/content/journal/acmi/10.1099/acmi.0.000535.v5
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