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Volume 4, Issue 9

Sorbitol non-fermenting and . O157: prevalence and antimicrobial resistance profile of strains in slaughtered food animals in Southeast Nigeria Open Access

Abstract

Antimicrobial resistance (AMR) is one of the greatest global health challenges. Reliable monitoring of AMR in bacteria in food animals is critical in order to devise mitigation strategies and empiric treatment of infections associated with the organisms. The aim of this study was to isolate sorbitol non-fermenting (SN-F) from food animals (broiler chickens, cattle and pigs) slaughtered at Ikpa Market, Nsukka, Enugu State, Southeast Nigeria, detect O157 and determine the antimicrobial resistance profile of the strains. A total of 388 faecal samples were collected from randomly selected broiler chickens (=155), cattle (=129) and pigs (=104). Isolation of SN-F was done using tryptic soy broth and cefixime- and potassium tellurite-supplemented sorbitol MacConkey agar (CT-SMAC). SN-F strains were confirmed biochemically and O157 detected serologically using specific O157 latex agglutination test kit. The antimicrobial resistance profile of all strains was established using the disc diffusion method. Overall, 52 (13.4 %) SN-F strains were recovered from the broiler chickens (=31), cattle (=12) and pigs (=9). A significant association (χ=9.70; <0.05) was observed between the prevalence of SN-F and animal species. O157 was detected in two of the samples, representing 0.51% of the 388 samples processed. All the strains were resistant to at least one antimicrobial agent tested and 76% were multidrug resistant (MDR). The mean multiple antibiotic resistance indices (MARI) for isolates from chickens, cattle and pig were 0.32, 0.18 and 0.23, respectively. This study showed that a considerable percentage of food animals slaughtered in Nsukka Southeastern Nigeria are potential reservoirs of multiple-drug-resistant SN-F including O157 that could spread to humans and the environment.

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  • Accepted:
  • Published Online:
Keyword(s): antimicrobial resistance , Escherichia coli O157 , food animals and sorbitol non-fermenting E. coli
Funding
This study was supported by the:
  • Tertiary Educational Trust Fund (TETFund) of the Nigerian government through the University of Nigeria, Nsukka Institution-Based Research (IBR) Intervention (Award TETFUND/DESS/UNN/NSUKKA/RP/VOL. VIV)
    • Principle Award Recipient: KennedyF. Chah
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2022-09-01
2024-04-23
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Sorbitol non-fermenting Escherichia coli and E. coli O157: prevalence and antimicrobial resistance profile of strains in slaughtered food animals in Southeast Nigeria
Access Microbiology 4, 000433 (2022); https://doi.org/10.1099/acmi.0.000433
/content/journal/acmi/10.1099/acmi.0.000433
/content/journal/acmi/10.1099/acmi.0.000433
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