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Volume 5, Issue 10

Identification of prosthetic joint infection by using metagenomic shotgun sequencing Open Access

Abstract

Periprosthetic joint infection (PJI) is a serious complication after total knee arthroplasty. Fungal infections are prone to biofilm formation, which makes it hard to diagnose and clarify the pathogenic species.

This case study provides evidence of a novel PJI pathogen that is otherwise difficult to detect using conventional methods. A patient was reviewed with persistent postoperative pain, swelling and eventually drainage around the left knee after undergoing a bilateral total knee arthroplasty 2 years previously for progressive osteoarthritis. By using metagenomic shotgun sequencing to analyse both bacterial and fungal agent sequences, we were able to identify fungal strains of , a rarely reported and difficult-to-culture PJI pathogen.

Metagenomic shotgun sequencing enables the detection of difficult-to-detect pathogens and the formulation of treatment recommendations for fungal infections with low positive rates based on gene content analysis.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Candida , metagenomic shotgun sequencing and prosthetic joint infection
Funding
This study was supported by the:
  • the Science and Technology Planning Project of Fuzhou (Award Grant 2019-SZ-16)
    • Principle Award Recipient: LiqiongLin
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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/content/journal/acmi/10.1099/acmi.0.000425
2023-10-26
2025-04-25
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/content/journal/acmi/10.1099/acmi.0.000425
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Identification of prosthetic joint infection by Candida using metagenomic shotgun sequencing
Access Microbiology 5, 000425 (2023); https://doi.org/10.1099/acmi.0.000425
/content/journal/acmi/10.1099/acmi.0.000425
/content/journal/acmi/10.1099/acmi.0.000425
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