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Abstract

We report the molecular evidence of dengue virus (DENV) and chikungunya virus (CHIKV) infection in symptomatic individuals in Cameroon and Gabon, respectively. Arthropod-borne viruses (arboviruses) are distributed in the tropical or subtropical regions, with DENV having the highest burden. The morbidity and mortality related to arboviral diseases raise the concern of timely and efficient surveillance and care. Our aim was to assess the circulation of arboviruses [DENV, CHIKV, Zika virus (ZIKV)] among febrile patients in Dschang (West Cameroon) and Kyé-ossi (South Cameroon, border with Gabon and Equatorial Guinea). Dried blood spots were collected from 601 consenting febrile patients, and 194 spp.-negative samples were tested for the molecular detection of cases of DENV, CHIKV and ZIKV infection. Overall, no case of ZIKV infection was found, whereas one case of DENV infection and one case of CHIKV infection were detected in Dschang and Kyé-ossi, respectively, with the CHIKV-infected patient being resident in Gabon. Our findings suggest the need to establish an active surveillance of arbovirus transmission in Cameroon and bordering countries.

Funding
This study was supported by the:
  • Sapienza Università di Roma (Award Disp n. 280/2017, Prot. N 0006599, 31 january 2017)
    • Principle Award Recipient: GhyslaineBruna Djeunang Dongho
  • Projet Intégré pour la Promotion de l'Auto-Développement
    • Principle Award Recipient: GhyslaineBruna Djeunang Dongho
  • Mingha Africa Onlus
    • Principle Award Recipient: GhyslaineBruna Djeunang Dongho
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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/content/journal/acmi/10.1099/acmi.0.000340
2022-04-29
2024-12-14
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References

  1. Gould EA, Solomon T. Pathogenic flaviviruses. Lancet 2008; 371:500–509 [View Article] [PubMed]
    [Google Scholar]
  2. Nemg Simo FB, Sado Yousseu FB, Evouna Mbarga A, Bigna JJ, Melong A et al. Investigation of an outbreak of dengue virus serotype 1 in a rural area of Kribi, South Cameroon: a cross-sectional study. Intervirology 2018; 61:265–271 [View Article]
    [Google Scholar]
  3. Simo FBN, Bigna JJ, Kenmoe S, Ndangang MS, Temfack E et al. Dengue virus infection in people residing in Africa: a systematic review and meta-analysis of prevalence studies. Sci Rep 2019; 9:13626 [View Article] [PubMed]
    [Google Scholar]
  4. Demanou M, Pouillot R, Grandadam M, Boisier P, Kamgang B et al. Evidence of dengue virus transmission and factors associated with the presence of anti-dengue virus antibodies in humans in three major towns in Cameroon. PLoS Negl Trop Dis 2014; 8:e2950 [View Article] [PubMed]
    [Google Scholar]
  5. Demanou M, Antonio-Nkondjio C, Ngapana E, Rousset D, Paupy C et al. Chikungunya outbreak in a rural area of Western Cameroon in 2006: A retrospective serological and entomological survey. BMC Res Notes 2010; 3:128 [View Article] [PubMed]
    [Google Scholar]
  6. Yousseu FBS, Nemg FBS, Ngouanet SA, Mekanda FMO, Demanou M. Association between dengue virus infection and, socio-demographic characteristics, population behavior and disease symptoms. Figshare 2018
    [Google Scholar]
  7. Maurice D, Alain S-MS, Christophe V, Rene N, Irene KT et al. Molecular characterization of chikungunya virus from three regions of Cameroon. Virol Sin 2015; 30:470–473 [View Article] [PubMed]
    [Google Scholar]
  8. Russo G, Subissi L, Rezza G. Chikungunya fever in Africa: a systematic review. Pathog Glob Health 2020; 114:136–144 [View Article] [PubMed]
    [Google Scholar]
  9. Liu ZY, Shi WF, Qin CF. The evolution of Zika virus from Asia to the Americas. Nat Rev Microbiol 2019; 17:131–139 [View Article] [PubMed]
    [Google Scholar]
  10. Aubry F, Jacobs S, Darmuzey M, Lequime S, Delang L et al. Recent African strains of Zika virus display higher transmissibility and fetal pathogenicity than Asian strains. Nat Commun 2021; 12:916 [View Article] [PubMed]
    [Google Scholar]
  11. Yuill TM. The ecology of tropical arthropod-borne viruses. Annu Rev Ecol Syst 1986; 17:189–219 [View Article]
    [Google Scholar]
  12. Tedjou AN, Kamgang B, Yougang AP, Njiokou F, Wondji CS. Update on the geographical distribution and prevalence of Aedes aegypti and Aedes albopictus (Diptera: Culicidae), two major arbovirus vectors in Cameroon. PLoS Negl Trop Dis 2019; 13:e0007137 [View Article] [PubMed]
    [Google Scholar]
  13. Barbara MAT, Liliane MT, Olivia A-A, Olivier TDS, Jean CM et al. The assessment of fever in under-five children in the Ekounou Health Area of Yaounde, Cameroon: Usefulness of rapid diagnostic tests. Int J Med Med Sci 2017; 9:33–40 [View Article]
    [Google Scholar]
  14. Birhanu Z, Abebe L, Sudhakar M, Dissanayake G, Yihdego YY-E et al. Malaria related perceptions, care seeking after onset of fever and anti-malarial drug use in malaria endemic settings of Southwest Ethiopia. PLoS One 2016; 11:1–20 [View Article] [PubMed]
    [Google Scholar]
  15. Hertz JT, Munishi OM, Sharp JP, Reddy EA, Crump JA. Comparing actual and perceived causes of fever among community members in a low malaria transmission setting in northern Tanzania. Trop Med Int Health 2013; 18:1406–1415 [View Article] [PubMed]
    [Google Scholar]
  16. Kassile T, Lokina R, Mujinja P, Mmbando BP. Determinants of delay in care seeking among children under five with fever in Dodoma region, central Tanzania: a cross-sectional study. Malar J 2014; 13:1–10 [View Article] [PubMed]
    [Google Scholar]
  17. Yousseu FBS, Nemg FBS, Ngouanet SA, Mekanda FMO, Demanou M. Detection and serotyping of dengue viruses in febrile patients consulting at the New-Bell District Hospital in Douala, Cameroon. PLoS One 2018; 13:e0204143 [View Article] [PubMed]
    [Google Scholar]
  18. Djeunang Dongho GB, Gunalan K, L’Episcopia M, Paganotti GM, Menegon M et al. Plasmodium vivax infections detected in a large number of febrile duffy-negative Africans in Dschang, Cameroon. Am J Trop Med Hyg 2021; 104:987–992 [View Article] [PubMed]
    [Google Scholar]
  19. Santiago GA, Vázquez J, Courtney S, Matías KY, Andersen LE et al. Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses. Nat Commun 2018; 9:1391 [View Article] [PubMed]
    [Google Scholar]
  20. Drosten C, Göttig S, Schilling S, Asper M, Panning M et al. Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J Clin Microbiol 2002; 40:2323–2330 [View Article] [PubMed]
    [Google Scholar]
  21. Rezza G, Nicoletti L, Angelini R, Romi R, Finarelli AC et al. Infection with chikungunya virus in Italy: an outbreak in a temperate region. Lancet 2007; 370:1840–1846 [View Article] [PubMed]
    [Google Scholar]
  22. Edwards CJ, Welch SR, Chamberlain J, Hewson R, Tolley H et al. Molecular diagnosis and analysis of Chikungunya virus. J Clin Virol 2007; 39:271–275 [View Article] [PubMed]
    [Google Scholar]
  23. Panning M, Grywna K, van Esbroeck M, Emmerich P, Drosten C. Chikungunya fever in travelers returning to Europe from the Indian Ocean region, 2006. Emerg Infect Dis 2008; 14:416–422 [View Article] [PubMed]
    [Google Scholar]
  24. Yenchitsomanus PT, Sricharoen P, Jaruthasana I, Pattanakitsakul SN, Nitayaphan S et al. Rapid detection and identification of dengue viruses by polymerase chain reaction (PCR). Southeast Asian J Trop Med Public Health 1996; 27:228–236 [PubMed]
    [Google Scholar]
  25. Krippner R, von Laer G. First confirmed dengue-1 fever cases reported from Cameroon. J Travel Med 2002; 9:273–274 [View Article] [PubMed]
    [Google Scholar]
  26. Tchuandom SB, Tchouangueu TF, Antonio-Nkondjio C, Lissom A, Djang JON et al. Seroprevalence of dengue virus among children presenting with febrile illness in some public health facilities in Cameroon. Pan Afr Med J 2018; 31:177 [View Article] [PubMed]
    [Google Scholar]
  27. Weetman D, Kamgang B, Badolo A, Moyes CL, Shearer FM et al. Aedes mosquitoes and aedes-borne arboviruses in africa: current and future threats. Int J Environ Res Public Health 2018; 15:E220 [View Article] [PubMed]
    [Google Scholar]
  28. Peyrefitte CN, Bessaud M, Pastorino BAM, Gravier P, Plumet S et al. Circulation of Chikungunya virus in Gabon, 2006-2007. J Med Virol 2008; 80:430–433 [View Article] [PubMed]
    [Google Scholar]
  29. Peyrefitte CN, Rousset D, Pastorino BAM, Pouillot R, Bessaud M et al. Chikungunya virus, Cameroon, 2006. Emerg Infect Dis 2007; 13:768–771 [View Article] [PubMed]
    [Google Scholar]
  30. Mohan A, Kiran DHN, Manohar IC, Kumar DP. Epidemiology, clinical manifestations, and diagnosis of Chikungunya fever: lessons learned from the re-emerging epidemic. Indian J Dermatol 2010; 55:54–63 [View Article] [PubMed]
    [Google Scholar]
  31. Monamele GC, Demanou M. First documented evidence of dengue and malaria co-infection in children attending two health centers in Yaoundé, Cameroon. Pan Afr Med J 2018; 29:227 [View Article] [PubMed]
    [Google Scholar]
  32. Nkenfou CN, Fainguem N, Dongmo-Nguefack F, Yatchou LG, Kameni JJK et al. Enhanced passive surveillance dengue infection among febrile children: Prevalence, co-infections and associated factors in Cameroon. PLoS Negl Trop Dis 2021; 15:e0009316 [View Article] [PubMed]
    [Google Scholar]
  33. Salam N, Mustafa S, Hafiz A, Chaudhary AA, Deeba F et al. Global prevalence and distribution of coinfection of malaria, dengue and chikungunya: a systematic review. BMC Public Health 2018; 18:710 [View Article] [PubMed]
    [Google Scholar]
  34. Raut CG, Rao NM, Sinha DP, Hanumaiah H, Manjunatha MJ. Chikungunya, dengue, and malaria co-infection after travel to Nigeria, India. Emerg Infect Dis 2015; 21:908–909 [View Article] [PubMed]
    [Google Scholar]
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