%0 Journal Article %A Lewis, Isabel C. %A Yan, Yao %A Finnigan, Gregory C. %T Analysis of a Cas12a-based gene-drive system in budding yeast %D 2021 %J Access Microbiology, %V 3 %N 12 %@ 2516-8290 %C 000301 %R https://doi.org/10.1099/acmi.0.000301 %K yeast %K gene drive %K Cas12a %K CRISPR %I Microbiology Society, %X The discovery and adaptation of CRISPR/Cas systems within molecular biology has provided advances across biological research, agriculture and human health. Genomic manipulation through use of a CRISPR nuclease and programmed guide RNAs has become a common and widely accessible practice. The identification and introduction of new engineered variants and orthologues of Cas9 as well as alternative CRISPR systems such as the type V group have provided additional molecular options for editing. These include distinct PAM requirements, staggered DNA double-strand break formation, and the ability to multiplex guide RNAs from a single expression construct. Use of CRISPR/Cas has allowed for the construction and testing of a powerful genetic architecture known as a gene drive within eukaryotic model systems. Our previous work developed a drive within budding yeast using Streptococcus pyogenes Cas9. Here, we installed the type V Francisella novicida Cas12a (Cpf1) nuclease gene and its corresponding guide RNA to power a highly efficient artificial gene drive in diploid yeast. We examined the consequence of altering guide length or introduction of individual mutational substitutions to the crRNA sequence. Cas12a-dependent gene-drive function required a guide RNA of at least 18 bp and could not tolerate most changes within the 5′ end of the crRNA. %U https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000301