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Volume 3, Issue 12

Detection of invasive in patient blood by a fungal PCR array Open Access

Abstract

Rare invasive fungal infections are increasingly emerging in hosts with predisposing factors such as immunodeficiency. Their timely diagnosis remains difficult, as their clinical picture may initially mimic infections with more common fungal species and species identification may be difficult with routine methods or may require time-consuming subcultures. This often results in ineffective drug administration and fatal outcomes. We report on a patient in their early twenties with mixed cellularity classical Hodgkin lymphoma with a disseminated () infection. Even though pathogen detection and identification was possible via the standard procedure consisting of culture followed by matrix-assisted laser desorption ionisation–time of flight (MALDI-TOF) mass spectrometry, the patient passed away in the course of multi organ failure. Herein, we report on a retrospectively applied experimental diagnostic fungal PCR-analysis used on an EDTA blood sample and consisting of two pan-fungal reactions and seven branch-specific reactions. Regarding invasive infection, this PCR array could considerably shorten time to diagnosis and switch to a targeted therapy with triazoles.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): fungal infections , hematologic malignancies , Hodgkin lymphoma , PCR and sepsis
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. The Microbiology Society waived the open access fees for this article.
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2021-12-03
2024-04-19
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Detection of invasive Trichosporon asahii in patient blood by a fungal PCR array
Access Microbiology 3, 000285 (2021); https://doi.org/10.1099/acmi.0.000285
/content/journal/acmi/10.1099/acmi.0.000285
/content/journal/acmi/10.1099/acmi.0.000285
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