RT Journal Article SR Electronic(1) A1 Godfrey, Anja A1 Osborn, Kay A1 Sinclair, Alison J.YR 2021 T1 Interaction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells JF Access Microbiology, VO 3 IS 11 OP SP 000282 DO https://doi.org/10.1099/acmi.0.000282 PB Microbiology Society, SN 2516-8290, AB Epstein–Barr virus (EBV) is present in a state of latency in infected memory B-cells and EBV-associated lymphoid and epithelial cancers. Cell stimulation or differentiation of infected B-cells and epithelial cells induces reactivation to the lytic replication cycle. In each cell type, the EBV transcription and replication factor Zta (BZLF1, EB1) plays a role in mediating the lytic cycle of EBV. Zta is a transcription factor that interacts directly with Zta response elements (ZREs) within viral and cellular genomes. Here we undertake chromatin-precipitation coupled to DNA-sequencing (ChIP-Seq) of Zta-associated DNA from cancer-derived epithelial cells. The analysis identified over 14 000 Zta-binding sites in the cellular genome. We assessed the impact of lytic cycle reactivation on changes in gene expression for a panel of Zta-associated cellular genes. Finally, we compared the Zta-binding sites identified in this study with those previously identified in B-cells and reveal substantial conservation in genes associated with Zta-binding sites., UL https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000282