1887

Abstract

We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75 % concordant overall and 80 % concordant for samples with high antibody levels. This demonstrates that commercial surrogate SARS-CoV-2 assays offer the potential to assess anti-SARS-CoV-2 antibodies’ neutralizing capacity outside CL-3 laboratory containment.

  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
Loading

Article metrics loading...

/content/journal/acmi/10.1099/acmi.0.000257
2021-08-26
2024-07-25
Loading full text...

Full text loading...

/deliver/fulltext/acmi/3/8/acmi000257.html?itemId=/content/journal/acmi/10.1099/acmi.0.000257&mimeType=html&fmt=ahah

References

  1. Mathuria JP, Yadav R R. Laboratory diagnosis of SARS-CoV-2 - A review of current methods. J Infect Public Health 202013901–905 [View Article] [PubMed]
    [Google Scholar]
  2. Caeseele PV. Network for the CPHL Bailey D. Chemists for the CS of C, Forgie SE, Canada for the A of MM and ID SARS-CoV-2 (COVID-19) serology: implications for clinical practice, laboratory medicine and public health. CMAJ 2020; 192:E973–9 [View Article] [PubMed]
    [Google Scholar]
  3. Maeda A, Maeda J. Review of diagnostic plaque reduction neutralization tests for flavivirus infection. Vet J 2013; 95:33–40 [View Article]
    [Google Scholar]
  4. Zakhartchouk AN, Liu Q, Petric M, Babiuk LA. Augmentation of immune responses to SARS coronavirus by a combination of DNA and whole killed virus vaccines. Vaccine 2005; 23:4385–4391 [View Article] [PubMed]
    [Google Scholar]
  5. Skowronski DM, Hottes TS, Janjua NZ, Purych D, Sabaiduc S et al. Prevalence of seroprotection against the pandemic (H1N1) virus after the 2009 pandemic. CMAJ 2010; 182:1851–1856 [View Article] [PubMed]
    [Google Scholar]
  6. Valcourt EJ, Kathy Manguiat K, Robinson A. Evaluation of a commercially-available surrogate virus neutralization test for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2. Diagn Microbiol Infect Dis 2021; 99: [View Article]
    [Google Scholar]
  7. Luchsinger LL, Ransegnola BP, Jin DK, Muecksch F, Weisblum Y et al. Serological assays estimate highly variable SARS-COV-2 neutralizing antibody activity in recovered COVID-19 patients. J Clin Microbiol 2020; 58: [View Article] [PubMed]
    [Google Scholar]
  8. Meyer B, Reimerink J, Torriani G, Brouwer F, Godeke G-J et al. Validation and clinical evaluation of a SARS-CoV-2 surrogate virus neutralisation test (sVNT. Emerg Microbes Infect 2020; 9:2394–2403 [View Article]
    [Google Scholar]
  9. Jiang S, Hillyer C, Du L. Neutralizing antibodies against SARS-COV-2 and other human coronaviruses. Trends Immunol 2020; 41:355–359 [View Article] [PubMed]
    [Google Scholar]
  10. Poh CM, Carissimo G, Wang B, Amrun SN, Lee CY-P et al. Two linear epitopes on the SARS-COV-2 spike protein that elicit neutralising antibodies in COVID-19 patients. Nat Commun 2020; 11:2806 [View Article] [PubMed]
    [Google Scholar]
  11. Ni L, Ye F, Cheng M-L, Feng Y, Deng Y-Q et al. Detection of SARS-CoV-2-specific humoral and cellular immunity in COVID-19 convalescent individuals. Immunity 2020; 52:971–977 [View Article] [PubMed]
    [Google Scholar]
  12. Prévost J, Gasser R, Beaudoin-Bussières G, Richard J, Duerr R et al. Cross-sectional evaluation of humoral responses against SARS-COV-2 spike. Cell Rep Med 2020; 1:100126 [View Article] [PubMed]
    [Google Scholar]
  13. Abe KT, Li Z, Samson R, Samavarchi-Tehrani P, Valcourt EJ et al. A simple protein-based surrogate neutralization assay for SARS-CoV-2. JCI Insight 2020; 5: [View Article]
    [Google Scholar]
  14. Bewley KR, Coombes NS, Gagnon L, McInroy L, Baker N et al. Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays. Nature Protocols 20211–33
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/acmi/10.1099/acmi.0.000257
Loading
/content/journal/acmi/10.1099/acmi.0.000257
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error