@article{mbs:/content/journal/acmi/10.1099/acmi.0.000249, author = "Wahyono, Daniel Joko and Khoeri, Mifathuddin Majid and Darmawan, Anton Budhi and Wijayanti, Siwi Pramatama Mars and Mumpuni, Aris and Nawangtantri, Gita and Kusdaryanto, Wahyu Dwi and Salsabila, Korrie and Safari, Dodi", title = "Nasopharyngeal carriage rates and serotype distribution of Streptococcus pneumoniae among school children with acute otitis media in Central Java, Indonesia", journal= "Access Microbiology", year = "2021", volume = "3", number = "7", pages = "", doi = "https://doi.org/10.1099/acmi.0.000249", url = "https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000249", publisher = "Microbiology Society", issn = "2516-8290", type = "Journal Article", keywords = "nasopharyngeal carriage", keywords = "serotype", keywords = "Streptococcus pneumoniae", keywords = "acute otitis media", eid = "000249", abstract = " Streptococcus pneumoniae is a common bacterial pathogen that causes acute otitis media (AOM) in children. In this study, we investigated nasopharyngeal carriage rates and serotype distributions of S. pneumoniae among school children with AOM in Banyumas Regency, Central Java, Indonesia, from 2018 to 2019. Nasopharyngeal swab specimens and demographic data were collected from 122 children between the ages of 6 and 12. The specimens were cultured for the identification of S. pneumoniae , and serotyping was performed using a sequential multiplex PCR assay. We found that the S. pneumoniae carriage rate was 73 % (89/122) among children with AOM. Serotypes 23A (11 %) and 6A/6B (10 %) were the most common serotypes among the 91 cultured S. pneumoniae strains, followed by 3 (8 %), 14 (7 %), 6C/6D (7 %), 11A/11D (6 %), 15B/15C (4 %) and 35 B (4 %). Moreover, 41 % of the strains could be covered by the 13-valent pneumococcal conjugate vaccine, PCV13. In conclusion, high nasopharyngeal carriage rates of S. pneumoniae were found in school children with AOM, with almost half of the strains being the vaccine-type. This finding provides a baseline for nasopharyngeal carriage of S. pneumoniae in school children with AOM and supports the implementation of pneumococcal conjugate vaccines in Indonesia.", }