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Abstract

Human bocavirus 1 (HBoV1) of the family causes mild to life-threatening respiratory tract infections in young children, but, due to widespread immunity, it is uncommon in adults. HBoV1 reinfections or reactivations leading to casualties are rare, but might be underdiagnosed. We report two young adults, one previously healthy and one immunosuppressed, with rare diagnostic patterns of HBoV1 respiratory tract infection. Both patients exhibited very high loads of HBoV1 DNA in respiratory samples. The immunosuppressed patient was also HBoV1 DNA-positive in blood, stool and a colon biopsy, but exhibited prior HBoV1-specific high-avidity IgG and weak IgM positivity 9 months before the respiratory symptoms. Likewise, the previously healthy patient exhibited HBoV1 IgG of high avidity and very weak IgM in serum, pointing to prior immunity, but with a seroconversion in cerebrospinal fluid. This patient also showed strong HBoV2 cross-reactivity. The molecular and serological results, together with their ages, suggest that both patients exhibited unusual reinfection or reactivation of HBoV1, contributing to neurological deficits and death.

Funding
This study was supported by the:
  • Svenska Kulturfonden
    • Principle Award Recipient: MariaSöderlund-Venermo
  • the Deutsche Forschungsgemeinschaft (Award Transregio 221)
    • Principle Award Recipient: DanielWolff
  • the Life and Health Medical Association Finland
    • Principle Award Recipient: MariaSöderlund-Venermo
  • Sigrid Juséliuksen Säätiö
    • Principle Award Recipient: MariaSöderlund-Venermo
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2021-07-08
2021-07-29
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References

  1. Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A et al. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A 2005; 102:12891–12896 [View Article] [PubMed]
    [Google Scholar]
  2. Qiu J, Söderlund-Venermo M, Young NS. Human parvoviruses. Clin Microbiol Rev 2017; 30:43–113 [View Article] [PubMed]
    [Google Scholar]
  3. Christensen A, Kesti O, Elenius V, Eskola AL, Døllner H et al. Human bocaviruses and paediatric infections. Lancet Child Adolesc Health 2019; 3:418–426 [View Article] [PubMed]
    [Google Scholar]
  4. Kapoor A, Simmonds P, Slikas E, Li L, Bodhidatta L et al. Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. J Infect Dis 2010; 201:1633–1643 [View Article] [PubMed]
    [Google Scholar]
  5. Kantola K, Hedman L, Tanner L, Simell V, Mäkinen M et al. B-cell responses to human bocaviruses 1–4: new insights from a childhood follow-up study. PLoS One 2015; 10:e0139096 [View Article] [PubMed]
    [Google Scholar]
  6. Guo L, Wang Y, Zhou H, Wu C, Song J et al. Differential seroprevalence of human bocavirus species 1-4 in Beijing, China. PLoS One 2012; 7:e39644
    [Google Scholar]
  7. Martin ET, Kuypers J, McRoberts JP, Englund JA, Zerr DM. Human bocavirus 1 primary infection and shedding in infants. J Infect Dis 2015; 212:516–524 [View Article] [PubMed]
    [Google Scholar]
  8. Jula A, Waris M, Kantola K, Peltola V, Söderlund-Venermo M et al. Primary and secondary human bocavirus 1 infections in a family, Finland. Emerg Infect Dis 2013; 19:1328 [View Article] [PubMed]
    [Google Scholar]
  9. Sadeghi M, Kantola K, Finnegan DP, McCaughey C, Hedman L et al. Possible involvement of human bocavirus 1 in the death of a middle-aged immunosuppressed patient. J Clin Microbiol 2013; 51:3461–3463 [View Article] [PubMed]
    [Google Scholar]
  10. Windisch W, Pieper M, Ziemele I, Rockstroh J, Brockmann M et al. Latent infection of human bocavirus accompanied by flare of chronic cough, fatigue and episodes of viral replication in an immunocompetent adult patient, Cologne, Germany. JMM Case Rep 2016; 3:e005052 [View Article] [PubMed]
    [Google Scholar]
  11. Kantola K, Sadeghi M, Antikainen J, Kirveskari J, Delwart E et al. Real-time quantitative PCR detection of four human bocaviruses. J Clin Microbiol 2010; 48:4044–4050 [View Article] [PubMed]
    [Google Scholar]
  12. Enders M, Lindner J, Wenzel JJ, Baisch C, Schalasta G et al. No detection of human bocavirus in amniotic fluid samples from fetuses with hydrops or isolated effusions. J Clin Virol 2009; 45:300–303 [View Article] [PubMed]
    [Google Scholar]
  13. Hedman L, Söderlund-Venermo M, Jartti T, Ruuskanen O, Hedman K. Dating of human bocavirus infection with protein-denaturing IgG-avidity assays—secondary immune activations are ubiquitous in immunocompetent adults. J Clin Virol 2010; 48:44–48 [View Article] [PubMed]
    [Google Scholar]
  14. Hedman K, Lappalainen M, Söderlund M, Hedman L. Avidity of IgG in serodiagnosis of infectious diseases. Rev Med Microbiol 1993; 4:123–129 [View Article]
    [Google Scholar]
  15. Fry AM, Lu X, Chittaganpitch M, Peret T, Fischer J et al. Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J Infect Dis 2007; 195:1038–1045 [View Article] [PubMed]
    [Google Scholar]
  16. Garbino J, Soccal PM, Aubert JD, Rochat T, Meylan P et al. Respiratory viruses in bronchoalveolar lavage: a hospital-based cohort study in adults. Thorax 2009; 64:399–404 [View Article] [PubMed]
    [Google Scholar]
  17. Aronen M, Viikari L, Kohonen I, Vuorinen T, Hämeenaho M et al. Respiratory tract virus infections in the elderly with pneumonia. BMC Geriatr 2019; 19:111 [View Article]
    [Google Scholar]
  18. Windisch W, Schildgen V, Malecki M, Lenz J, Brockmann M et al. Detection of HBoV DNA in idiopathic lung fibrosis, Cologne, Germany. J Clin Virol 2013; 58:325–327 [View Article] [PubMed]
    [Google Scholar]
  19. Krakau M, Gerbershagen K, Frost U, Hinzke M, Brockmann M et al. Case report: human bocavirus associated pneumonia as cause of acute injury, Cologne, Germany. Medicine (Baltimore) 2015; 94:e1587 [View Article] [PubMed]
    [Google Scholar]
  20. Mitui MT, Shahnawaz Bin TS, Matsumoto T, Khanam W, Ahmed S et al. Detection of human bocavirus in the cerebrospinal fluid of children with encephalitis. Clin Infect Dis 2012; 54:964–967 [View Article] [PubMed]
    [Google Scholar]
  21. Yu JM, Chen QQ, Hao YX, Yu T, Zeng SZ et al. Identification of human bocaviruses in the cerebrospinal fluid of children hospitalized with encephalitis in China. J Clin Virol 2013; 57:374–377
    [Google Scholar]
  22. Mori D, Ranawaka U, Yamada K, Rajindrajith S, Miya K et al. Human bocavirus in patients with encephalitis, Sri Lanka, 2009–2010. Emerg Infect Dis 2013; 19:1859 [View Article] [PubMed]
    [Google Scholar]
  23. Akturk H, Sık G, Salman N, Sutcu M, Tatli B et al. Atypical presentation of human bocavirus: severe respiratory tract infection complicated with encephalopathy. J Med Virol 2015; 87:1831–1838 [View Article] [PubMed]
    [Google Scholar]
  24. Söderlund-Venermo M, Lahtinen A, Jartti T, Hedman L, Kemppainen K et al. Clinical assessment and improved diagnosis of bocavirus-induced wheezing in children, Finland. Emerg Infect Dis 2009; 15:1423 [View Article] [PubMed]
    [Google Scholar]
  25. Xu M, Arku B, Jartti T, Koskinen J, Peltola V et al. Comparative diagnosis of human bocavirus 1 respiratory infection with messenger RNA reverse-transcription polymerase chain reaction (PCR), DNA quantitative PCR, and serology. J Infect Dis 2017; 215:1551–1557 [View Article] [PubMed]
    [Google Scholar]
  26. Söderlund M, Ruutu P, Ruutu T, Asikainen K, Franssila R et al. Primary and secondary infections by human parvovirus B19 following bonemarrow transplantation: characterization by PCR and B-cell molecular immunology. Scand J Infect Dis 1997; 29:129–135 [View Article] [PubMed]
    [Google Scholar]
  27. Kainulainen L, Waris M, Söderlund-Venermo M, Allander T, Hedman K et al. Hepatitis and human bocavirus primary infection in a child with T-cell deficiency. J Clin Microbiol 2008; 46:4104–4105 [View Article] [PubMed]
    [Google Scholar]
  28. Li X, Kantola K, Hedman L, Arku B, Hedman K et al. Original antigenic sin with human bocaviruses 1-4. J Gen Virol 2015; 96:3099–3108 [View Article] [PubMed]
    [Google Scholar]
  29. Vatti A, Monsalve DM, Pacheco Y, Chang C, Anaya JM et al. Original antigenic sin: a comprehensive review. J Autoimmun 2017; 83:12–21 [View Article] [PubMed]
    [Google Scholar]
  30. Xu M, Perdomo MF, Mattola S, Pyöriä L, Toppinen M et al. Persistence of human bocavirus 1 in tonsillar germinal centers and antibody-dependent enhancement of infection. mBio 2021; 12:e03132-20 [View Article] [PubMed]
    [Google Scholar]
  31. Sinclair W, Omar M. Enterovirus. In StatPearls [Internet] Treasure Island (FL: StatPearls Publishing; 2021
    [Google Scholar]
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